Literature summary for 5.4.2.8 extracted from

Qian, W.; Yu, C.; Qin, H.; Liu, X.; Zhang, A.; Johansen, I.E.; Wang, D.
Molecular and functional analysis of phosphomannomutase (PMM) from higher plants and genetic evidence for the involvement of PMM in ascorbic acid biosynthesis in Arabidopsis and Nicotiana benthamiana (2007), Plant J., 49, 399-413.

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Activating Compound

Activating Compound	Comment	Organism	Structure
D-Glucose 1,6-bisphosphate	in presence of 0.02 and 0.2 mM mannose 1-phosphate substrate, half-maximal stimulation of recombinant enzyme at 0.004 and 0.008 mM D-glucose 1,6-bisphosphate	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
-	Nicotiana benthamiana
expression in Escherichia coli with His-tag, expression as green fluorescent protein fusion protein in Arabidospsis thaliana	Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	expression as green fluorescent protein fusion protein in Arabidospsis thaliana increases ascorbic acid content by 25-33%	Arabidopsis thaliana
additional information	reducing enzyme expression level through virus-induced gene silencing causes substantial decrease in ascorbic acid content in leaves. Raising the expression level leads to 20-50% increase in ascorbic acid content	Nicotiana benthamiana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0297	-	D-mannose 1-phosphate	30°C, pH 7.5	Arabidopsis thaliana
0.0654	-	D-glucose 1-phosphate	30°C, pH 7.5	Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
27700	-	x * 27700, calculated and MALDI-TOF	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	O80840	-	-
Nicotiana benthamiana	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	enzyme is constitutively expressed in both vegetative and reproductive organs	Nicotiana benthamiana	-
additional information	enzyme is constitutively expressed in both vegetative and reproductive organs	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-glucose 1-phosphate	-	Nicotiana benthamiana	D-glucose 6-phosphate	-	?
D-glucose 1-phosphate	reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate	Arabidopsis thaliana	D-glucose 6-phosphate	-	?
D-Mannose 1-phosphate	-	Nicotiana benthamiana	D-Mannose 6-phosphate	-	r
D-Mannose 1-phosphate	reaction with mannose 1-phosphate is more efficient than with D-glucose-1-phosphate	Arabidopsis thaliana	D-Mannose 6-phosphate	-	r
D-mannose 6-phosphate	-	Nicotiana benthamiana	D-mannose 1-phosphate	-	r
D-mannose 6-phosphate	-	Arabidopsis thaliana	D-mannose 1-phosphate	-	r

Subunits

Subunits	Comment	Organism
?	x * 27700, calculated and MALDI-TOF	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	-	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.1	-	95% of maximum activity	Arabidopsis thaliana
7.5	-	-	Arabidopsis thaliana

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Release 2023.1 (January 2023)