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Literature summary for 5.4.2.7 extracted from
- Phosphopentomutase of Bacillus stearothermophilus TH6-2: the enzyme and its gene ppm (1998), Biosci. Biotechnol. Biochem., 62, 1103-1108.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43735 | - |
1 * 43735, calculation from nucleotide sequence | Geobacillus stearothermophilus |
| 43735 | - |
1 * 43735, deduced from nucleotide sequence | Geobacillus stearothermophilus |
| 48000 | - |
1 * 48000, SDS-PAGE | Geobacillus stearothermophilus |
| 53000 | - |
gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-ribose 1-phosphate | Geobacillus stearothermophilus | PPM is involve in the salvage pathway of nucleoside synthesis | D-ribose 5-phosphate | - |
? |  |
| additional information | Geobacillus stearothermophilus | the enzyme is involved in the salvage pathway of nucleoside synthesis | ? | - |
? | |
| additional information | Geobacillus stearothermophilus TH6-2 | the enzyme is involved in the salvage pathway of nucleoside synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Geobacillus stearothermophilus | - |
- |
- |
| Geobacillus stearothermophilus | - |
TH6-2 | - |
| Geobacillus stearothermophilus TH6-2 | - |
TH6-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant PPM, Toyopearl HW-55, DEAE-Toyopearl, Butyl-Toyopearl, Sephacryl S-330 | Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 41.7 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,3-dideoxyribose 5-phosphate | - |
Geobacillus stearothermophilus | 2,3-dideoxyribose 1-phosphate | - |
? | |
| 2,3-dideoxyribose 5-phosphate | - |
Geobacillus stearothermophilus | 2,3-dideoxyribose 1-phosphate | - |
r | |
| 2,3-dideoxyribose 5-phosphate | - |
Geobacillus stearothermophilus TH6-2 | 2,3-dideoxyribose 1-phosphate | - |
? | |
| 2-deoxy-D-ribose 5-phosphate | - |
Geobacillus stearothermophilus | 2-deoxy-D-ribose 1-phosphate | - |
r | |
| 2-deoxyribose 5-phosphate | - |
Escherichia coli | 2-deoxyribose 1-phosphate | - |
? | |
| 2-deoxyribose 5-phosphate | at 156% of the activity relative to ribose 5-phosphate | Geobacillus stearothermophilus | 2-deoxyribose 1-phosphate | - |
? | |
| 2-deoxyribose 5-phosphate | at 156% of the activity relative to ribose 5-phosphate | Geobacillus stearothermophilus TH6-2 | 2-deoxyribose 1-phosphate | - |
? | |
| alpha-D-ribose 1-phosphate | - |
Geobacillus stearothermophilus | D-ribose 5-phosphate | - |
r | |
| alpha-D-ribose 1-phosphate | PPM is involve in the salvage pathway of nucleoside synthesis | Geobacillus stearothermophilus | D-ribose 5-phosphate | - |
? | |
| arabinose 5-phosphate | - |
Geobacillus stearothermophilus | arabinose 1-phosphate | - |
r | |
| D-Arabinose 5-phosphate | - |
Escherichia coli | D-Arabinose 1-phosphate | - |
? | |
| D-Arabinose 5-phosphate | at 8.61% of the activity relative to ribose 5-phosphate | Geobacillus stearothermophilus | D-Arabinose 1-phosphate | - |
? | |
| D-Arabinose 5-phosphate | at 8.61% of the activity relative to ribose 5-phosphate | Geobacillus stearothermophilus TH6-2 | D-Arabinose 1-phosphate | - |
? | |
| D-ribose 1-phosphate | only the reverse reaction is mentioned: D-ribose 5-phosphate to D-ribose 1-phosphate | Escherichia coli | D-ribose 5-phosphate | - |
? | |
| D-ribose 1-phosphate | only the reverse reaction is mentioned: D-ribose 5-phosphate to D-ribose 1-phosphate | Geobacillus stearothermophilus | D-ribose 5-phosphate | - |
? | |
| D-ribose 1-phosphate | only the reverse reaction is mentioned: D-ribose 5-phosphate to D-ribose 1-phosphate | Geobacillus stearothermophilus TH6-2 | D-ribose 5-phosphate | - |
? | |
| additional information | the enzyme is involved in the salvage pathway of nucleoside synthesis | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | the enzyme is involved in the salvage pathway of nucleoside synthesis | Geobacillus stearothermophilus TH6-2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Escherichia coli |
| monomer | 1 * 48000, SDS-PAGE | Geobacillus stearothermophilus |
| monomer | 1 * 43735, calculation from nucleotide sequence | Geobacillus stearothermophilus |
| monomer | 1 * 43735, deduced from nucleotide sequence | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPM | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Escherichia coli |
| 65 | - |
ribose 1-phosphate synthesis, at pH 8.0 | Geobacillus stearothermophilus |
| 65 | - |
at pH 8.0 | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 6.0-9.0, 1 h, stable | Geobacillus stearothermophilus |
| 55 | - |
pH 8.0, 15 min, completely stable below | Geobacillus stearothermophilus |
| 55 | 70 | no loss of activity at 55°C at pH 8.0 after 15 min, complete loss of activity after 15 min at 70°C and pH 8.0 | Geobacillus stearothermophilus |
| 70 | - |
pH 8.0, 15 min, stable | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
at 50°C | Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 50°C, 1 h, stable | Geobacillus stearothermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Geobacillus stearothermophilus | isoelectric focusing | - |
4.85 |
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