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Literature summary for 5.4.2.6 extracted from

  • Zhang, G.; Dai, J.; Wang, L.; Dunaway-Mariano, D.; Tremblay, L.W.; Allen, K.N.
    Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis (2005), Biochemistry, 44, 9404-9416.
    View publication on PubMed
Search for more literature for 5.4.2.6

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with Mg2+ Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
D170A 700fold reduction of kcat-value Lactococcus lactis
D8A no catalytic activity Lactococcus lactis
D8E no catalytic activity Lactococcus lactis
E169A/D170A 1400fold reduction of kcat-value Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0078
-
 beta-D-glucose 1-phosphate mutant D170A, 25°C, pH 7.0 Lactococcus lactis
0.0146
-
 beta-D-glucose 1-phosphate wild-type, 25°C, pH 7.0 Lactococcus lactis
0.39
-
 beta-D-glucose 1-phosphate mutant E169A/D170A, 25°C, pH 7.0 Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Km-value 0.270 mM Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis P71447
-
-

Reaction

Reaction Comment Organism Reaction ID
beta-D-Glucose 1-phosphate = beta-D-glucose 6-phosphate a single Mg2+ coordination site accomodates water, phosphate, and the phosphorane intermediate during catalytic turnover. Bi-bi ping-pong mechanism, substrate induced-fit mechanism allows phosphomutase activity to dominate over the intrinsic phosphatase activity Lactococcus lactis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate
-
 Lactococcus lactis beta-D-Glucose 6-phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0012
-
 beta-D-glucose 1-phosphate mutant E169A/D170A, 25°C, pH 7.0 Lactococcus lactis
0.00384
-
 beta-D-glucose 1-phosphate mutant D170A, 25°C, pH 7.0 Lactococcus lactis
17.1
-
 beta-D-glucose 1-phosphate wild-type, 25°C, pH 7.0 Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Lactococcus lactis