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Literature summary for 5.4.2.6 extracted from

  • Lahiri, S.D.; Zhang, G.; Radstrom, P.; Dunaway-Mariano, D.; Allen, K.N.
    Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus (2002), Acta Crystallogr. Sect. D, 58, 324-326.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of native and selenomethionyl beta-PGM in escherichia coli Lactococcus lactis

Crystallization (Commentary)

Crystallization (Comment) Organism
beta-PGM is transferred to 1 mM HEPES, pH 7.5, 10 mM MgCl2 and 0.1 mM dithiothreitol, final beta-PGM concentration at 15 mg/ml, crystals are obtained from either 150 mM ammonium acetate, 100 mM trisodium citrate dihydrate, pH 4.5, 25% polyethylene glycol 4000 or 100 mM ammonium fluoride, 16% polyethylene glycol 3350 by hanging-drop vapor diffusion, crystals of selenium methionyl-substituted beta-PGM diffract to 2.3 A Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
beta-D-glucose 1-phosphate in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ Lactococcus lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Lactococcus lactis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-D-Glucose 1-phosphate Lactococcus lactis
-
beta-D-Glucose 6-phosphate
-
r

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant native and selenomethionyl beta-PGM Lactococcus lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18
-
native beta-PGM Lactococcus lactis
40
-
recombinant beta-PGM Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-Glucose 1-phosphate
-
Lactococcus lactis beta-D-Glucose 6-phosphate
-
r

Synonyms

Synonyms Comment Organism
beta-PGM
-
Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
17
-
beta-D-glucose 1-phosphate in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ Lactococcus lactis