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Literature summary for 5.4.2.4 extracted from

  • Ravel, P.; Craescus, C.T.; Arous, N.; Rosa, J.; Garel, M.C.
    Critical Role of Human bisphosphoglycerate mutase Cys22 in the phosphatase activator-binding site (1997), J. Biol. Chem., 272, 14045-14050.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of variants by site directed mutagenesis replacing Ser23 and Cys22 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.182
-
2,3-diphosphoglycerate mutant S23G Homo sapiens
0.281
-
2,3-diphosphoglycerate mutant C22S Homo sapiens
0.3
-
2,3-diphosphoglycerate wild-type Homo sapiens
0.583
-
2,3-diphosphoglycerate mutant C22T Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3-Diphosphoglycerate Homo sapiens in red blood cells 2,3-diphosphoglycerate is the main allosteric effector of hemoglobin, shifting the equilibrium between the oxy and deoxy conformations of hemoglobin preferentially stabilizing the unliganded form. 2,3-Diphosphoglycerate also promotes the polymerization of deoxyhemoglobin in sickle cell disease ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
Homo sapiens
-

Storage Stability

Storage Stability Organism
-80°C, sodium phosphate buffer, 20% glycerol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3-Diphosphoglycerate the enzyme has additional activities of EC 5.4.2.1(phosphoglycerate mutase) and EC 3.1.3.13 (bisphosphoglycerate phosphatase) Homo sapiens 2,3-Diphosphoglycerate
-
?
1,3-Diphosphoglycerate multifunctional enzyme for synthesis and degradation of 2,3-diphosphoglycerate Homo sapiens 2,3-Diphosphoglycerate
-
?
2,3-Diphosphoglycerate in red blood cells 2,3-diphosphoglycerate is the main allosteric effector of hemoglobin, shifting the equilibrium between the oxy and deoxy conformations of hemoglobin preferentially stabilizing the unliganded form. 2,3-Diphosphoglycerate also promotes the polymerization of deoxyhemoglobin in sickle cell disease Homo sapiens ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0037
-
2,3-diphosphoglycerate wild-type Homo sapiens
0.00623
-
2,3-diphosphoglycerate mutant C22S Homo sapiens
0.00845
-
2,3-diphosphoglycerate mutant S23G Homo sapiens
0.152
-
2,3-diphosphoglycerate mutant C22T Homo sapiens