General Stability | Organism |
---|---|
loss of activity is observed when phosphate buffer is used in the pH range of 6.0-8.0 | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00103 | - |
D-glucose 1,6-diphosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum | |
0.1 | - |
alpha-D-glucose 1-phosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme is also active with 5 mM Ca2+ but to a lesser extent as Co2+ (11.9% relative activity) | Fusarium oxysporum | |
Co2+ | PGM requires the presence of a divalent cation for its function with maximum activity (100%) being obtained with 5 mM Co2+ | Fusarium oxysporum | |
Mg2+ | the enzyme is also active with 5 mM Mg2+ but to a lesser extent as Co2+ (24.5% relative activity) | Fusarium oxysporum | |
Mn2+ | the enzyme is also active with 5 mM Mn2+ but to a lesser extent as Co2+ (69.5% relative activity) | Fusarium oxysporum | |
Ni2+ | the enzyme is also active with 5 mM Ni2+ but to a lesser extent as Co2+ (22.5% relative activity) | Fusarium oxysporum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
1 * 56000, SDS-PAGE | Fusarium oxysporum |
60000 | - |
gel filtration | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Fusarium oxysporum F3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Sephacryl-S 100 gel filtration | Fusarium oxysporum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
crude enzyme, at pH 7.6, 25°C | Fusarium oxysporum |
5.3 | - |
after 5fold purification, at pH 7.6, 25°C | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose 1-phosphate | - |
Fusarium oxysporum | alpha-D-glucose 6-phosphate | - |
? | |
alpha-D-glucose 1-phosphate | - |
Fusarium oxysporum F3 | alpha-D-glucose 6-phosphate | - |
? | |
D-glucose 1,6-diphosphate | - |
Fusarium oxysporum | ? | - |
? | |
D-glucose 1,6-diphosphate | - |
Fusarium oxysporum F3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Fusarium oxysporum |
Synonyms | Comment | Organism |
---|---|---|
PGM | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Fusarium oxysporum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 50 | - |
Fusarium oxysporum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | PGM is almost stable (12% loss of the activity) at 30°C for 60 min, while almost complete loss of activity is observed at 50 and 60°C after 10 min | Fusarium oxysporum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.74 | - |
alpha-D-glucose 1-phosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum | |
0.74 | - |
D-glucose 1,6-diphosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
using 3-(N-morpholino)propanesulfonic acid buffer | Fusarium oxysporum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | using 3-(N-morpholino)propanesulfonic acid buffer, the enzyme retains up to 90% of its initial activity | Fusarium oxysporum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | at pH 7.0 only 17.5% of the relative activity is recovered, the enzyme retains 14% of its initial activity after incubation at pH 10.0 | Fusarium oxysporum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fusarium oxysporum | isoelectric focusing | - |
3.5 |
General Information | Comment | Organism |
---|---|---|
metabolism | PGM is a key enzyme in carbohydrate metabolism, where it catalyzes the reversible transfer of a phosphate group between C-1 and C-6 of D-glucose via D-glucose 1,6-diphosphate | Fusarium oxysporum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.38 | - |
alpha-D-glucose 1-phosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum | |
714.3 | - |
D-glucose 1,6-diphosphate | in 41 mM 1,3-diaza-2,4-cyclopentadiene /HCl (pH 7.6), 5 mM MgCl2, at 25°C | Fusarium oxysporum |