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Literature summary for 5.4.2.2 extracted from
- Regulation of glucosylation of teichoic acid. I. Isolation of phosphoglucomutase in Bacillus subtilis 168 (1974), J. Biol. Chem., 249, 5169-5175.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-glucose 1,6-diphosphate | - |
Bacillus subtilis |  |
| EDTA | activation | Bacillus subtilis | |
| EGTA | activation | Bacillus subtilis | |
General Stability
| General Stability | Organism |
|---|---|
| extremely unstable in crude extracts, protease sensitive, Bacillus subtilis 168 SR 22 can be used as a protease-negative mutant | Bacillus subtilis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | Bacillus subtilis | glucosylation of teichoic acid | ? | - |
? | |
| Glucose 6-phosphate | Bacillus subtilis | glucosylation of teichoic acid | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 2 isozymes | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 31.5 | - |
substrate glucose 1-phosphate | Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crude enzyme extract, 0.2 mM EDTA, 10% glycerol, pH 6.5, 3-4 weeks stable | Bacillus subtilis |
| 4°C, purified enzyme, 75% saturated ammonium sulfate, 0.2 mM EDTA, 10% glycerol, pH 6.5, 3 months stable | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-D-glucose 1-phosphate | - |
Bacillus subtilis | alpha-D-glucose 6-phosphate | - |
? | |
| alpha-D-glucose 1-phosphate | glucosylation of teichoic acid | Bacillus subtilis | ? | - |
? | |
| Glucose 6-phosphate | - |
Bacillus subtilis | Glucose 1-phosphate | - |
? | |
| Glucose 6-phosphate | glucosylation of teichoic acid | Bacillus subtilis | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | - |
- |
Bacillus subtilis |
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