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Literature summary for 5.4.2.12 extracted from

  • Guerra, D.G.; Vertommen, D.; Fothergill-Gilmore, L.A.; Opperdoes, F.R.; Michels, P.A.
    Characterization of the cofactor-independent phosphoglycerate mutase from Leishmania mexicana mexicana. Histidines that coordinate the two metal ions in the active site show different susceptibilities to irreversible chemical modification (2004), Eur. J. Biochem., 271, 1798-1810.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with C-terminal His-tag Leishmania mexicana

General Stability

General Stability Organism
rapid decrease in activity when no stabilizer is added. In presence of NaCl, CoCl2, and imidazole, stable for 1 month at 4°C Leishmania mexicana

Inhibitors

Inhibitors Comment Organism Structure
diethyl dicarbonate irreversible inhibition, saturating concentrations of substrate protect Leishmania mexicana
EDTA 1 mM, 10% residual activity at pH 8.0, reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 Leishmania mexicana
additional information not inhibitory: vanadate, cloride, sulfate Leishmania mexicana
phosphate 100 mM, 70% residual activity Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
2-phospho-D-glycerate pH 7.6, 25°C Leishmania mexicana
0.27
-
3-phospho-D-glycerate pH 7.6, 25°C Leishmania mexicana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Leishmania mexicana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
CoCl2 inhibition by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4, NiCl2, ZnCl2 Leishmania mexicana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE Leishmania mexicana
60724
-
x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE Leishmania mexicana

Organism

Organism UniProt Comment Textmining
Leishmania mexicana Q86N96 sp. mexicana
-

Renatured (Commentary)

Renatured (Comment) Organism
inactivation by EDTA is reversible by incubation with CoCl2, but not MnCl2, FeSO4, CuSo4,NiCl2, ZnCl2 Leishmania mexicana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
419
-
formation of 2-phospho-D-glycerate, pH 7.6, 25°C Leishmania mexicana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phospho-D-glycerate
-
Leishmania mexicana 3-phospho-D-glycerate
-
r
3-phospho-D-glycerate
-
Leishmania mexicana 2-phospho-D-glycerate
-
r

Subunits

Subunits Comment Organism
? x * 60724, calculated, x * 60000, recombinant protein with His-tag, SDS-PAGE Leishmania mexicana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
199
-
2-phospho-D-glycerate pH 7.6, 25°C Leishmania mexicana
434
-
3-phospho-D-glycerate pH 7.6, 25°C Leishmania mexicana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.2 for both directions of reaction Leishmania mexicana

pH Range

pH Minimum pH Maximum Comment Organism
6.8 8.8 more than 75% of maximum activity within this range, formation of 2-phospho-D-glycerate Leishmania mexicana

Cofactor

Cofactor Comment Organism Structure
additional information 2,3-bisphosphoglycerate independent phosphoglycerate mutase Leishmania mexicana

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania mexicana calculated
-
5.26