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Literature summary for 5.4.2.11 extracted from

  • White, M.; Fothergill-Gilmore L.A.
    Mutase versus synthase: the phosphoglycerate mutase family studied by protein engineering (1990), Biochem. Soc. Trans., 18, 257.
    View publication on PubMed
Search for more literature for 5.4.2.11

Activating Compound

Activating Compound Comment Organism Structure
2,3-diphosphoglycerate Km: 0.005, wild-type enzyme, Km: 0.003, mutant K246G, Km: 0.048, mutant S11G Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K246G mutant enzymes in which Ser11 is replaced by Gly, i.e. S11G and Lys246 by Gly, i.e. K246G do not have significantly altered kinetic values Saccharomyces cerevisiae
S11G mutant enzymes in which Ser11 is replaced by Gly, i.e. S11G and Lys246 by Gly, i.e. K246G do not have significantly altered kinetic values Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
 2-phosphoglycerate wild-type enzyme Saccharomyces cerevisiae
0.06
-
 2-phosphoglycerate mutant K246G Saccharomyces cerevisiae
0.067
-
 2-phosphoglycerate mutant S11G Saccharomyces cerevisiae
0.65
-
 3-phosphoglycerate wild-type enzyme Saccharomyces cerevisiae
0.67
-
 3-phosphoglycerate mutant S11G Saccharomyces cerevisiae
0.68
-
 3-phosphoglycerate mutant K246G Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-Phosphoglycerate Saccharomyces cerevisiae glycolysis/gluconeogenesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Reaction

Reaction Comment Organism Reaction ID
2-phospho-D-glycerate = 3-phospho-D-glycerate mechanism: after the active site His-8 is phosphorylated by the cofactor 2,3-diphosphoglycerate a substrate molecule binds. Subsequently a phospho-transfer takes place via a ping-pong-mechanism. After release of the product the enzyme remains phosphorylated and catalytically competent Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-Phosphoglycerate
-
 Saccharomyces cerevisiae 3-Phosphoglycerate
-
 ?
3-Phosphoglycerate
-
 Saccharomyces cerevisiae 2-Phosphoglycerate
-
 ?
3-Phosphoglycerate glycolysis/gluconeogenesis Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 3-phosphoglycerate mutant S11G Saccharomyces cerevisiae
324
-
 3-phosphoglycerate mutant K246G Saccharomyces cerevisiae
384
-
 3-phosphoglycerate wild-type enzyme Saccharomyces cerevisiae