Application | Comment | Organism |
---|---|---|
synthesis | the highly stereospecific enzyme can be used in a biotransformation system producing (R)-styrene oxide | Corynebacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, 29% inhibition | Corynebacterium sp. | |
dithiothreitol | 1 mM, 22% inhibition | Corynebacterium sp. | |
HgCl2 | 1 mM, 80% inhibition | Corynebacterium sp. | |
hydrazine | 1 mM, 82% inhibition | Corynebacterium sp. | |
hydroxylammonium chloride | 1 mM, 77% inhibition | Corynebacterium sp. | |
iodoacetate | 1 mM, 80% inhibition | Corynebacterium sp. | |
KCN | 1 mM, 32% inhibition | Corynebacterium sp. | |
PCMB | 1 mM, 24% inhibition | Corynebacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
styrene oxide | pH 7.0, 25°C | Corynebacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | not membrane-bound, probably bound to the cell wall | Corynebacterium sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | non-metal enzyme | Corynebacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene oxide + H2O | Corynebacterium sp. | key enzyme of styrene and styrene oxide metabolism | phenylacetaldehyde | - |
ir | |
styrene oxide + H2O | Corynebacterium sp. AC-5 | key enzyme of styrene and styrene oxide metabolism | phenylacetaldehyde | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Corynebacterium sp. AC-5 | - |
- |
- |
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. SK3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | cells grown in a styrene gas atmosphere | Corynebacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
58.7 | - |
- |
Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
styrene oxide + H2O | compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer | Corynebacterium sp. | phenylacetaldehyde | - |
? | |
styrene oxide + H2O | key enzyme of styrene and styrene oxide metabolism | Corynebacterium sp. | phenylacetaldehyde | - |
ir | |
styrene oxide + H2O | compared with the (R)-isomer, the relative activity of the enzyme towards (R)-styrene oxide is 51% of that for the (S)-isomer | Corynebacterium sp. AC-5 | phenylacetaldehyde | - |
? | |
styrene oxide + H2O | key enzyme of styrene and styrene oxide metabolism | Corynebacterium sp. AC-5 | phenylacetaldehyde | - |
ir |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Corynebacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.0, 1 h, stable | Corynebacterium sp. |
50 | - |
pH 7.0, 1 h, 10% loss of activity | Corynebacterium sp. |
55 | - |
pH 7.0, 1 h, 50% loss of activity | Corynebacterium sp. |
60 | - |
pH 7.0, 1 h, 80% loss of activity | Corynebacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | - |
Corynebacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | pH 5.0-7.5: maximal activity, pH 10.0: about 50% of maximal activity | Corynebacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
25°C, 1 h, unstable below | Corynebacterium sp. |
7 | 8 | 25°C, 1 h | Corynebacterium sp. |
10 | - |
25°C, 1 h, 10% loss of activity | Corynebacterium sp. |