Literature summary for 5.3.99.6 extracted from

Lu, X.; Zhang, F.; Shen, Q.; Jiang, W.; Pan, Q.; Lv, Z.; Yan, T.; Fu, X.; Wang, Y.; Qian, H.; Tang, K.
Overexpression of allene oxide cyclase improves the biosynthesis of artemisinin in Artemisia annua L (2014), PLoS ONE, 9, e91741.

Cloned(Commentary)

Cloned (Comment)	Organism
cloning of the promoter of AaAOC, the key gene of jasmonate biosynthetic pathway, expression analysis, AaAOC gene overexpression under control of the 35S promoter in Artemisia annua leading to an increase of 1.6 to 5.2fold in enzyme expression and 2 to 4.7fold increase in jasmonate levels, as well as to a significant increase of contents of artemisinin, dihydroartemisinic acid and artemisinic acid, expression analysis by RT-Q-PCR	Artemisia annua

Cloned (Comment)

Organism

cloning of the promoter of AaAOC, the key gene of jasmonate biosynthetic pathway, expression analysis, AaAOC gene overexpression under control of the 35S promoter in Artemisia annua leading to an increase of 1.6 to 5.2fold in enzyme expression and 2 to 4.7fold increase in jasmonate levels, as well as to a significant increase of contents of artemisinin, dihydroartemisinic acid and artemisinic acid, expression analysis by RT-Q-PCR

Artemisia annua

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate	Artemisia annua	-	(15Z)-12-oxophyto-10,15-dienoate	-	?

Organism

Organism	UniProt	Comment	Textmining
Artemisia annua	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
flower bud	-	Artemisia annua	-
leaf	-	Artemisia annua	-
additional information	ubiquitous expression, expression pattern analysis, overview	Artemisia annua	-
root	-	Artemisia annua	-
stem	-	Artemisia annua	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate	-	Artemisia annua	(15Z)-12-oxophyto-10,15-dienoate	-	?

Synonyms

Synonyms	Comment	Organism
AaAOC	-	Artemisia annua
Allene oxide cyclase	-	Artemisia annua

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Artemisia annua

General Information

General Information	Comment	Organism
metabolism	key enzyme of the jasmonate biosynthetic pathway catalysing the formation of (9S,13S)-12-oxo-phytodienoic acid and establishes the stereochemical configuration of naturally occurring jasmonate. Spontaneous hydrolysis of the allene oxide also yields (9S,13S)-12-oxo-phytodienoic acid, although in its racemic form	Artemisia annua
additional information	contents of artemisinin, dihydroartemisinic acid and artemisinic acid, as well as jasmonate levels and expression levels of farnesyl diphosphate synthase, cytochrome P450 dependent hydroxylase, and double bond reductase 2, are increased significantly in AaAOC overexpression Artemisia annua plants	Artemisia annua