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Literature summary for 5.3.99.6 extracted from

  • Kong, F.J.; Li, Y.; Abe, J.; Liu, B.; Schaller, F.; Piotrowski, M.; Otagaki, S.; Takahashi, K.; Matsuura, H.; Yoshihara, T.; Nabeta, K.
    Expression of allene oxide cyclase from Pharbitis nil upon theobroxide treatment (2009), Biosci. Biotechnol. Biochem., 73, 1007-1013.
    View publication on PubMed
Search for more literature for 5.3.99.6

Activating Compound

Activating Compound Comment Organism Structure
theobroxide
-
 Ipomoea nil

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEM-T Easy for sequencing and subsequently into the vector pQE30 for expression in Escherichia coli M15 cells Ipomoea nil

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Ipomoea nil 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20660
-
 theoretical, without putative chloroplast signal peptide Ipomoea nil
21000
-
 recombinant protein, determined by SDS-PAGE and Western blot analysis Ipomoea nil
26160
-
 theoretical Ipomoea nil

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate Ipomoea nil
-
 (9S,13S,15Z)-12-oxophyto-10,15-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Ipomoea nil Q2PP43
-
-

Purification (Commentary)

Purification (Comment) Organism
on a Ni-NTA column Ipomoea nil

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Ipomoea nil
-
vascular bundle
-
 Ipomoea nil
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(9Z,13S,15Z)-12,13-epoxyoctadeca-9,11,15-trienoate
-
 Ipomoea nil (9S,13S,15Z)-12-oxophyto-10,15-dienoate
-
 ?

Synonyms

Synonyms Comment Organism
Allene oxide cyclase
-
 Ipomoea nil
PnAOC
-
 Ipomoea nil

pI Value

Organism Comment pI Value Maximum pI Value
Ipomoea nil theoretical, without putative chloroplast signal peptide
-
 6.19
Ipomoea nil theoretical
-
 8.9

General Information

General Information Comment Organism
metabolism allene oxide cyclase is the most important enzyme for the jasmonic acid biosynthesis Ipomoea nil