Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | the catalytic activities of the binary phosphatidylethanolamine/phosphatidylcholine and phosphatidylserine/phosphatidylcholine and ternary phosphatidylcholine/phosphatidylethanolamine/phosphatidylserine are similar and increase relative to the unary phosphatidylcholine system | Homo sapiens | |
phosphatidylcholine | - |
Homo sapiens | |
phosphatidylethanolamine | - |
Homo sapiens | |
phosphatidylserine | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of C-terminally His-tagged enzyme replacing the first 29 amino acid residues in the N-terminal transmembrane domain with the hydrophilic sequence MAKKTSS for TXAS cDNA in Escherichia coli strain DH5alpha | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum membrane | associated | Homo sapiens | 5789 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | cyanide binding to substrate-free and substrate-bound CYP5A1 in a detergent-solubilized system and in nanodiscs, binding kinetics, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | Homo sapiens | - |
(5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged modified enzyme from Escherichia coli strain DH5alpha by nickel affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate | - |
Homo sapiens | (5Z,13E)-(15S)-9alpha,11alpha-epoxy-15-hydroxythromboxa-5,13-dienoate | - |
? | |
additional information | substrate binding analysis using substrate analogues of PGH2, U44069 and U46619 | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP5A1 | - |
Homo sapiens |
thromboxane synthase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | role of membranes in modulating the thermodynamics and kinetics of enzyme substrate binding, redox potential of enzyme incorporated into lipid bilayers of nanodiscs, overview. Binding of substrate to the enzyme can induce conformational changes in the protein that block small-molecule ligand egress | Homo sapiens |
physiological function | the enzyme is a membrane-associated cytochrome P450 that metabolizes the cyclooxygenase product prostaglandin into thromboxane A2, a potent inducer of vasoconstriction and platelet aggregation | Homo sapiens |