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Literature summary for 5.3.99.4 extracted from

  • Yeh, H.C.; Hsu, P.Y.; Wang, J.S.; Tsai, A.L.; Wang, L.H.
    Characterization of heme environment and mechanism of peroxide bond cleavage in human prostacyclin synthase (2005), Biochim. Biophys. Acta, 1738, 121-132.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0133
-
 prostaglandin H2
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the recombinant enzyme associates with host cell membranes Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Prostaglandin H2 Homo sapiens
-
 Prostaglandin I2
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to electrophoretic homogeneity Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46
-
 CM column, assayed at 23°C using 30 microM prostaglandin H1 as the substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
10-hydroperoxyoctadeca-8,12-dienoic acid
-
 Homo sapiens 10-oxooctadeca-8,12-dienoic acid + 10-oxodec-8-enoic acid + 10-hydroxyoctadeca-8,12-dienoic acid
-
 ?
prostaglandin H1
-
 Homo sapiens 12-hydroxy-8,10-heptadecadienoic acid + malondialdehyde
-
 ?
Prostaglandin H2
-
 Homo sapiens Prostaglandin I2
-
 ?

Synonyms

Synonyms Comment Organism
PGIS
-
 Homo sapiens
prostacyclin synthase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
heme heme/protein ratio is 0.9:1 Homo sapiens