Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
DELTA-20-PGIS | PGH2 presentation to the membrane-bound mutant has a 2 to 3fold delay compared to that of membrane-bound wild-type PGIS | Homo sapiens |
PGIS/TXAS(8-27) | the first 20 membrane anchor residues of human PGIS are replaced by the corresponding membrane anchor region of human TXAS (thromboxane A2 synthase). Efficiency of the isomerization of PGH2 to PGI2 is slowed down with approximately 25, 65, and 85% at 30, 60, and 120 s reactions, compared to the wild-type PGIS, respectively | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
chimer PGIS/TXAS(8-27), monomer, SDS-PAGE | Homo sapiens |
57000 | - |
mutant delta-20-PGIS, monomer, SDS-PAGE | Homo sapiens |
57000 | - |
wild-type, monomer, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | Homo sapiens | in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2 | Prostaglandin I2 | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | within 30 s, about 75% of PGH2 is presented to the PGIS active side and isomerized to PGI2 by the membrane-bound PGIS. In contrast, only about 20% of the substrate is isomerized to the product by the detergent-solubilized (Triton X-100) PGIS. Replacement of the membrane with detergent does not alter the PGIS active side conformation, but affects the substrate presentation | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | - |
Homo sapiens | Prostaglandin I2 | - |
? | |
Prostaglandin H2 | in vivo, this reaction is coupled to a previous one, where arachidonic acid is converted by cyclooxygenase and yields the substrate PGH2 | Homo sapiens | Prostaglandin I2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGIS | - |
Homo sapiens |
prostacyclin synthase | - |
Homo sapiens |
Prostaglandin I2 synthase | - |
Homo sapiens |