Literature summary for 5.3.99.4 extracted from

Hara, S.; Miyata, A.; Yokoyama, C.; Inoue, H.; Brugger, R.; Lottspeich, F.; Ullrich, V.; Tanabe, T.
Isolation and molecular cloning of prostacyclin synthase from bovine endothelial cells (1994), J. Biol. Chem., 269, 19897-19903.

Search for more literature for 5.3.99.4

Cloned(Commentary)

Cloned (Comment)	Organism
-	Bos taurus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0073	-	prostaglandin H2	-	Bos taurus
0.009	-	prostaglandin H2	-	Bos taurus
0.0094	-	Prostaglandin H1	-	Bos taurus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
52000	-	x * 52000, SDS-PAGE	Bos taurus
56628	-	x * 56628, calculation from nucleotide sequence	Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Prostaglandin H2	Bos taurus	formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Bos taurus	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
endothelium	-	Bos taurus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2	-	-	Bos taurus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
prostaglandin H1	-	Bos taurus	12-hydroxyheptadecadienoic acid + malondialdehyde	-	?
Prostaglandin H2	-	Bos taurus	Prostaglandin I2	prostaglandin I2	?
Prostaglandin H2	-	Bos taurus	Prostaglandin I2	prostaglandin I2 is readily hydrolyzed to 6-keto-prostaglandin F1alpha	?
Prostaglandin H2	formation of prostaglandin I2, which is a powerful vasodilator and the most potent natural occuring inhibitor of platelet aggregation	Bos taurus	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 52000, SDS-PAGE	Bos taurus
?	x * 56628, calculation from nucleotide sequence	Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.45	-	prostaglandin H2	-	Bos taurus
26.5	-	Prostaglandin H1	-	Bos taurus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)