Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C65A/C167A | site-directed mutagenesis, the mutant still provides the disulfide bond between Cys89 and Cys186 | Homo sapiens |
W43F/C65A/C167A | site-directed mutagenesis, the W43F mutant cannot be purified owing to the inclusion body formation of protein | Homo sapiens |
W54F/C65A/W112F/C167A | site-directed mutagenesis, the mutant still provides the disulfide bond between Cys89 and Cys186 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the lipid transporter activity of the enzyme is competitively inhibited by pamitate, stearate and arachnoate | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the N-terminal 22-amino acid residues correspond to the putative secretion signal peptide of the enzyme | Homo sapiens | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41222 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, gel filtration, and dialysis | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cerebrospinal fluid | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
Beta-trace protein | - |
Homo sapiens |
L-PGDS | - |
Homo sapiens |
lipocalin-type PG D synthase | - |
Homo sapiens |
lipocalin-type prostaglandin d synthase | - |
Homo sapiens |
Prostaglandin-H2 D-isomerase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
purified recombinant enzyme, sodium phosphate, pH 7.0, containing 5% v/v ethanol, stable up to | Homo sapiens |
65 | 90 | purified recombinant enzyme, thermal unfolding in the absence and presence of small lipophilic ligands, two-state unfolding transition, overview | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the lipocalin superfamily | Homo sapiens |
physiological function | lipocalin-type prostaglandin D synthase is a multi-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid-transporter. It is involved in the biosynthesis of prostaglandin D2, acting as an endogenous somnogen and allergy response. Binding mechanism of small lipophilic molecules by the enzyme functioning as lipid transporter, e.g. of biliverdin, all-trans-retinoic acid, L-thyroxine (T4), progesterone, and genistein, thermodynamic parameters, overview. The enzme shows broad ligand selectivity for small lipophilic molecules, it binds heme metabolites with significantly higher affinity than other small lipophilic ligands | Homo sapiens |