Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C65A/C167A | site-directed mutagenesis | Homo sapiens |
C89A/C167A/C186A | site-directed mutagenesis | Homo sapiens |
C89A/C186A | site-directed mutagenesis | Homo sapiens |
additional information | knockdown of the enzyme expression by siRNA in SH-SY5Y cells, two different siRNAs | Homo sapiens |
Y63S/T67S/C89A/C186A | site-directed mutagenesis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | rough endoplasmic reticulum membrane | Homo sapiens | 5783 | - |
extracellular | secreted to the cerebrospinal fluid | Homo sapiens | - |
- |
membrane | rough endoplasmic reticulum membrane | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | lipocalin-type prostaglandin D synthase is a dual-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid transporter, the enzyme can bind a wide variety of lipophilic molecules | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41222 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
arachnoid mater | - |
Homo sapiens | - |
brain | synthesized mainly at the rough endoplasmic reticulum membrane of arachnoid cells, chorioid plexus cells and oligodendrocytes, and then is secreted into the cerebrospinal fluid as a second major protein | Homo sapiens | - |
cerebrospinal fluid | second major protein | Homo sapiens | - |
choroid plexus | - |
Homo sapiens | - |
neuron | - |
Homo sapiens | - |
oligodendrocyte | - |
Homo sapiens | - |
SH-SY5Y cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | lipocalin-type prostaglandin D synthase is a dual-functional protein, acting as a prostaglandin D2-producing enzyme and a lipid transporter, the enzyme can bind a wide variety of lipophilic molecules | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure homology modelling using the solution structure of the C65A mouse L-PGDS, PDB code 2RQ0, as template, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-PGDS | - |
Homo sapiens |
lipocalin-type PGD synthase | - |
Homo sapiens |
lipocalin-type prostaglandin d synthase | - |
Homo sapiens |
Prostaglandin D synthase | - |
Homo sapiens |
Prostaglandin-H2 D-isomerase | UniProt name | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | enzyme expression is increased in H2O2-treated neuronal cells | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the lipocalin superfamily | Homo sapiens |
additional information | structure homology modelling using the solution structure of the C65A mouse L-PGDS, PDB code 2RQ0, as template, overview | Homo sapiens |
physiological function | the enzyme shows a protective effect on H2O2-induced apoptosis in neuroblastoma cell line SH-SY5Y, the enzyme level is highly associated with H2O2-induced apoptosis. It protects against neuronal cell death by scavenging reactive oxygen species without losing its ligand-binding function. H2O2 reacts with the thiol of Cys65 of the enzyme | Homo sapiens |