Cloned (Comment) | Organism |
---|---|
mutant C65A is expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant C65A in complex with oleic acid and palmitoleic acid, hanging drop vapor diffusion method, using 0.1 M citric acid (pH 4.0) and 1.7 M ammonium sulfate | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C65A | inactive | Homo sapiens |
F83A | the mutation reduces the catalytic efficiency by almost 10fold | Homo sapiens |
K59A | the mutation enhances the catalytic efficiency by more than 2fold | Homo sapiens |
L131A | the mutation reduces the catalytic efficiency by almost 10fold | Homo sapiens |
L79A | the mutation reduces the catalytic efficiency by almost 10fold | Homo sapiens |
M64A | the mutation reduces the catalytic efficiency by almost 10fold | Homo sapiens |
M94A | the mutation reduces the catalytic efficiency | Homo sapiens |
S45A | the mutation reduces the catalytic efficiency | Homo sapiens |
S81A | the mutation reduces the catalytic efficiency | Homo sapiens |
T147A | the mutation slightly enhances the catalytic efficiency | Homo sapiens |
W54A | the mutation reduces the catalytic efficiency | Homo sapiens |
Y149A | the mutation enhances the catalytic efficiency by more than 2fold | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0102 | - |
prostaglandin H2 | mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0123 | - |
prostaglandin H2 | mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0127 | - |
prostaglandin H2 | mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0138 | - |
prostaglandin H2 | wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0154 | - |
prostaglandin H2 | mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0161 | - |
prostaglandin H2 | mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0202 | - |
prostaglandin H2 | mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0204 | - |
prostaglandin H2 | mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0245 | - |
prostaglandin H2 | mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0252 | - |
prostaglandin H2 | mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0325 | - |
prostaglandin H2 | mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.0328 | - |
prostaglandin H2 | mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P41222 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Superdex G75 gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Prostaglandin H2 | - |
Homo sapiens | Prostaglandin D2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
monomer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-PGDS | - |
Homo sapiens |
L-prostaglandin D synthase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
prostaglandin H2 | mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.63 | - |
prostaglandin H2 | mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.81 | - |
prostaglandin H2 | mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
0.87 | - |
prostaglandin H2 | mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2.23 | - |
prostaglandin H2 | mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2.24 | - |
prostaglandin H2 | mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2.4 | - |
prostaglandin H2 | mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2.64 | - |
prostaglandin H2 | wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2.91 | - |
prostaglandin H2 | mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
3.67 | - |
prostaglandin H2 | mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
5.73 | - |
prostaglandin H2 | mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
5.84 | - |
prostaglandin H2 | mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
110 | - |
prostaglandin H2 | mutant enzyme L79A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
1170 | - |
prostaglandin H2 | mutant enzyme M64A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
1890 | - |
prostaglandin H2 | mutant enzyme L131A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
2120 | - |
prostaglandin H2 | mutant enzyme F83A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
6570 | - |
prostaglandin H2 | mutant enzyme S45A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
6660 | - |
prostaglandin H2 | mutant enzyme M94A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
8940 | - |
prostaglandin H2 | mutant enzyme W54A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
9420 | - |
prostaglandin H2 | mutant enzyme S81A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
11490 | - |
prostaglandin H2 | wild type enzyme, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
14290 | - |
prostaglandin H2 | mutant enzyme T147A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
24840 | - |
prostaglandin H2 | mutant enzyme K59A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens | |
27050 | - |
prostaglandin H2 | mutant enzyme Y149A, in 100 mM Tris-HCl (pH 8.0), 100 mM NaCl, and 1 mM dithiothreitol, at 25°C | Homo sapiens |