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Literature summary for 5.3.99.2 extracted from

  • Kumasaka, T.; Aritake, K.; Ago, H.; Irikura, D.; Tsurumura, T.; Yamamoto, M.; Miyano, M.; Urade, Y.; Hayaishi, O.
    Structural basis of the catalytic mechanism operating in open-closed conformers of lipocalin type prostaglandin D synthase (2009), J. Biol. Chem., 284, 22344-22352.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.99.2

Cloned(Commentary)

Cloned (Comment) Organism
for expression in Escherichia coli cells Mus musculus

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant DELTA1-24_C65A L-PGDS is crystallized in two different crystal forms representing the conformational change between the open and closed states of the cavity of the beta-barrel, the structures are determined to resolutions of 2.1 and 2.0 A Mus musculus

Protein Variants

Protein Variants Comment Organism
DELTA1-24_C65A mutant Mus musculus
DELTA1-24_C65A+H111A mutant Mus musculus
DELTA1-24_C65A+P110A mutant Mus musculus
DELTA1-24_C65A+W54A mutant Mus musculus
DELTA1-24_L-PGDS mutant Mus musculus
DELTA1-24_L-PGDS+H111A mutant Mus musculus
DELTA1-24_L-PGDS+H116A mutant Mus musculus
DELTA1-24_L-PGDS+P110A mutant Mus musculus
DELTA1-24_L-PGDS+S45A mutant Mus musculus
DELTA1-24_L-PGDS+S45A/S81A mutant Mus musculus
DELTA1-24_L-PGDS+S45A/T67A mutant Mus musculus
DELTA1-24_L-PGDS+S45A/T67A/S81A mutant Mus musculus
DELTA1-24_L-PGDS+S81A mutant Mus musculus
DELTA1-24_L-PGDS+T67A mutant Mus musculus
DELTA1-24_L-PGDS+T67A/S81A mutant Mus musculus
DELTA1-24_L-PGDS+W45A mutant Mus musculus
DELTA1-24_L-PGDS+W54A/H111A mutant Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
U-46619
-
 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+S45A Mus musculus
0.0008
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS Mus musculus
0.0008
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+W54A/H111A Mus musculus
0.0012
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+S45A/S81A Mus musculus
0.0012
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+S45A/T67A/S81A Mus musculus
0.0013
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+W45A Mus musculus
0.0014
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+H111A Mus musculus
0.0014
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+H116A Mus musculus
0.0015
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+S81A Mus musculus
0.0016
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+P110A Mus musculus
0.0021
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+S45A/T67A Mus musculus
0.0021
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+T67A/S81A Mus musculus
0.0028
-
 prostaglandin H2 mutant DELTA1-24_L-PGDS+T67A Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Prostaglandin H2 Mus musculus
-
 Prostaglandin D2
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus O09114
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Prostaglandin H2
-
 Mus musculus Prostaglandin D2
-
 ?

Synonyms

Synonyms Comment Organism
L-PGDS
-
 Mus musculus
lipocalin type prostaglandin D synthase
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 activity assay Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Mus musculus

General Information

General Information Comment Organism
physiological function lipocalin type prostaglandin D synthase is a multi-functional protein acting as a somnogen (PGD2)-producing enzyme, an extracellular transporter of various lipophilic ligands, and an amyloid-beta chaperone in human cerebrospinal fluid Mus musculus