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Literature summary for 5.3.99.2 extracted from

  • Inui, T.; Ohkubo, T.; Urade, Y.; Hayaishi, O.
    Enhancement of lipocalin-type prostaglandin D synthase enzyme activity by guanidine hydrochloride (1999), Biochem. Biophys. Res. Commun., 266, 641-646.
    View publication on PubMed
Search for more literature for 5.3.99.2

Activating Compound

Activating Compound Comment Organism Structure
guanidinium hydrochloride 2.5fold activation at 500 mM, inhibition above, less than 5% activity at 2 M guanidinium hydrochloride Mus musculus
guanidinium hydrochloride activation between 100-1000 mM, 3fold activation at 500 mM guanidinium hydrochloride, inhibition above 500 mM, less than 5% activity at 3 M Homo sapiens
guanidinium hydrochloride activation up to 750 mM, 1.5fold activition at 500 mM, inhibition above 1 M, complete deactivation at 4 M Mus musculus
Urea 1.6fold activation at 1M, inhibition above, less than 5% activity at 4 M urea Mus musculus
Urea activation between 250-2000 mM, 1.7fold activation at 1 M urea, inhibition above 500 mM, less than 5% activity at 4 M Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mus musculus
expression of cDNA in Escherichia coli Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0023
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate in the presence of 1 M urea Mus musculus
0.0028
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Mus musculus
0.0083
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate in the presence of 500 mM guanidinium hydrochloride Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Homo sapiens
-
 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
 ?
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate Mus musculus
-
 (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus O09114
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
complete recovery of activity after denaturation with 2 M guanidinium hydrochloride or 4 M urea Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Homo sapiens (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
 ?
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Mus musculus (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate
-
 ?

Synonyms

Synonyms Comment Organism
lipocalin-type prostaglandin d synthase
-
 Mus musculus
lipocalin-type prostaglandin d synthase i.e. L-PGDS Homo sapiens
lipocalin-type prostaglandin d synthase i.e. L-PGDS Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.37
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate
-
 Mus musculus
0.5
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate in the presence of 1 M urea Mus musculus
0.95
-
 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate in the presence of 500 mM guanidinium hydrochloride Mus musculus