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Literature summary for 5.3.4.1 extracted from
- Entamoeba histolytica: biochemical characterization of a protein disulfide isomerase (2011), Exp. Parasitol., 128, 76-81.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | PDI represents a potential drug target for anti-amebic therapy | Entamoeba histolytica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli XL1-Blue MRF' cells | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| bacitracin | - |
Entamoeba histolytica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0116 | - |
scrambled reoxidized lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
| 0.0253 | - |
denatured-reduced lysozyme | pH 7.0, temperature not specified in the publication | Entamoeba histolytica | |
| 2.026 | - |
Insulin | pH 7.0, temperature not specified in the publication | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA-agarose column chromatography, gel filtration | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| denatured-reduced lysozyme | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| insulin | reductase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| additional information | PDI behaves mainly as an oxidase/isomerase and exhibits chaperone-like activity | Entamoeba histolytica | ? | - |
? | |
| RNase A | oxidase activity of PDI | Entamoeba histolytica | ? | - |
? | |
| scrambled reoxidized lysozyme | isomerase activity of PDI | Entamoeba histolytica | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDI | - |
Entamoeba histolytica |
| protein disulfide isomerase | - |
Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
pH optimum for the reductase activity of recombinant PDI | Entamoeba histolytica |
| 8 | - |
pH optimum for the oxidase activity of recombinant PDI | Entamoeba histolytica |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.172 | - |
using denatured-reduced lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
| 0.334 | - |
using scrambled reoxidized lysozyme as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
| 1.137 | - |
using insulin as substrate, at pH 7.0, temperature not specified in the publication | Entamoeba histolytica | bacitracin | |
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Release 2023.1 (January 2023)
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