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Literature summary for 5.3.4.1 extracted from
- Neospora caninum: Functional inhibition of protein disulfide isomerase by the broad-spectrum anti-parasitic drug nitazoxanide and other thiazolides (2008), Exp. Parasitol., 118, 80-88.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged enzyme in Escherichia coli | Neospora caninum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoic acid | - |
Neospora caninum |  |
| bacitracin | - |
Neospora caninum | |
| nitazoxanide | a broad-spectrum anti-parasitic drug | Neospora caninum | |
| nitazoxanide thiazolide derivatives | PDI is inhibited by those thiazolides that also affected parasite proliferation | Neospora caninum | |
| tizoxanide | deacetylated metabolite of nitazoxanide | Neospora caninum | |
| tocinoic acid | - |
Neospora caninum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
x * 52000, native enzyme, SDS-PAGE, x * 55000, recombinant His-tagged enzyme, SDS-PAGE | Neospora caninum |
| 55000 | - |
x * 52000, native enzyme, SDS-PAGE, x * 55000, recombinant His-tagged enzyme, SDS-PAGE | Neospora caninum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neospora caninum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from tachyzoit extracts by tizoxanide affinity chromatography, recombinant His-tagged enzyme from Escherichia coli by Co2+ affinity chromatography | Neospora caninum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tachyzoite | - |
Neospora caninum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| insulin | reduction of disulfide bonds | Neospora caninum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52000, native enzyme, SDS-PAGE, x * 55000, recombinant His-tagged enzyme, SDS-PAGE | Neospora caninum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDI | - |
Neospora caninum |
| protein disulfide isomerase | - |
Neospora caninum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Neospora caninum |
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Release 2023.1 (January 2023)
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