Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type PDI and mutant abb'a' lacking the C-terminal domain c in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | under thermally denaturing conditions of 80°C or at 1 M guanidine-HCl the wild-type PDI is still active in renaturing LDH, while the mutant abb'a', lacking the C-terminal domain c, loses its chaperone function | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | light scattering and fluorescence measurement kinetics of wild-type PDI and mutant abb'a' lacking the C-terminal domain c, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type PDI and mutant abb'a' from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactate dehydrogenase | reactivation of self-aggregated denatured lactate dehydrogenase, guanidine HCl-denatured LDH, chaperone activity, both recombinant wild-type PDI and mutant abb'a' interact with self-aggregated lactate dehydrogenase enhancing LDH reactivation and reducing aggregation | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | PDI domain organization, 5 domains a, b, b', a', and c, the acidic C-terminal domain c, residues 463-491, stabilizes the chaperone function of the enzyme | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PDI | - |
Homo sapiens |
protein disulfide isomerase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |