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Literature summary for 5.3.4.1 extracted from

  • Urban, A.; Leipelt, M.; Eggert, T.; Jaeger, K.E.
    DsbA and DsbC affect extracellular enzyme formation in Pseudomonas aeruginosa (2001), J. Bacteriol., 183, 587-596.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.4.1

Cloned(Commentary)

Cloned (Comment) Organism
genes dsbA and dsbC, DNA sequence determination and analysis, expression of His-tagged DsbA and DsbC in Escherichia coli strain BL(DE3) Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
C34S site-directed mutagenesis, the mutant shows impaired lipase activity, the defect cannot be rescued by constitutive expression of lipA gene encoding lipase A Pseudomonas aeruginosa
additional information construction of mutants deficient in either DsbA or DsbC, or both, the dsbA mutant does no longer produce extracellular enzymes elastase, alkaline phosphatase, and lipase, while in the dcbC mutant the lipase activity is doubled, introduction of dsbC into the dsbA mutant results in low extracellular lipase activity, overview Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
 2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE Pseudomonas aeruginosa
25000
-
 2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa DsbA and DsbC are involved in disulfide bond formation and play an important role in the formation of extracellular enzymes, DsbA is important in lipase stability and excretion ?
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
 genes dsbA and dsbC
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged DsbA and DsbC from Escherichia coli strain BL(DE3) by nickel affinity chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DsbA and DsbC are involved in disulfide bond formation and play an important role in the formation of extracellular enzymes, DsbA is important in lipase stability and excretion Pseudomonas aeruginosa ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 22000, DsbA, SDS-PAGE, 2 * 25000, DsbC, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
DsbA
-
 Pseudomonas aeruginosa
DsbC
-
 Pseudomonas aeruginosa