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Literature summary for 5.3.4.1 extracted from

  • Kainuma, K.; Ookura, T.; Kawamura, Y.
    Purification and characterization of protein disulfide isomerase from soybean (1995), J. Biochem., 117, 208-215.
    View publication on PubMed
Search for more literature for 5.3.4.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 40% inhibition Glycine max
E-64 0.01 mM, 11% inhibition after treatment with 0.01 mM DTT. No inhibition without DTT Glycine max
iodoacetate 1 mM, almost complete inhibition after reduction with 0.01 mM DTT. No inhibition in absence of DTT Glycine max
Mg2+ 1 mM, 20% inhibition Glycine max
Zn2+ 1 mM, 70% inhibition Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 2 * 63000, SDS-PAGE Glycine max
120000
-
 gel filtration Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Glycine max

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Proteins native, reduced or with wrong disulfide bonds Glycine max Proteins with correct disulfide bonds ?
Proteins incorrectly disulfide-linked Bowman Birk soybean trypsin Glycine max Proteins with correct disulfide bonds ?
Proteins incorrectly disulfide-linked lysozyme Glycine max Proteins with correct disulfide bonds ?
Proteins incorrectly disulfide-linked RNAse Glycine max Proteins with correct disulfide bonds ?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 7.5, 1 h, stable up to Glycine max
60
-
 pH 7.5, 1 h, about 45% loss of activity Glycine max
80
-
 pH 7.5, 1 h, about 70% loss of activity Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7: about 50% of maximal activity, pH 9: about 60% of maximal activity Glycine max

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 1 h, 0°C or 37°C, highest stability Glycine max