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Literature summary for 5.3.3.5 extracted from
- Purification, characterization and catalytic properties of human sterol 8-isomerase (2002), Biochem. J., 367, 587-599.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in erg2 Saccharomyces cerevisiae and in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 25-Azacholesterol | - |
Homo sapiens |  |
| cholesterol | - |
Homo sapiens | |
| emopamil | - |
Homo sapiens | |
| ketoconazole | - |
Homo sapiens | |
| tamoxifen | - |
Homo sapiens | |
| tridemorph | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
Zymosterol | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26700 | - |
4 * 26700, MALDI-TOF mass spectrometry | Homo sapiens |
| 27000 | - |
4 * 27000, SDS-PAGE | Homo sapiens |
| 107000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q15125 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.243 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude enzyme preparation, stable for 2 months | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 26,27-dehydrozymosterol | - |
Homo sapiens | 5beta-cholest-7-en-3beta-ol | - |
? | |
| 26,27-dinorcholesta-8,24-dienol | - |
Homo sapiens | 26,27-dinorcholesta-7,24-dienol | - |
? | |
| 26-homo-cholesta-8(9),23(24) E26(26')-trienol | - |
Homo sapiens | 26-homo-cholesta-7(8),23(24)E26(26')trienol | - |
? | |
| 5alpha-cholesta-8,24-dien-3beta-ol | - |
Homo sapiens | 5alpha-cholesta-7,24-dien-3beta-ol | - |
? | |
| cholest-8-enol | - |
Homo sapiens | cholest-7-enol | - |
? | |
| fecosterol | - |
Homo sapiens | 24-methylene-5alpha-cholest-7-en-3beta-ol | - |
? | |
| additional information | 3-desoxyzymosterol is not an active substrate | Homo sapiens | ? | - |
? | |
| zymosterol | preferred substrate for DELTA7-sterol production, the reaction equilibrium for double-bond isomerization favours the forward direction, DELTA8 to DELTA7 | Homo sapiens | cholesta-7,24-dienol | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 27000, SDS-PAGE | Homo sapiens |
| tetramer | 4 * 26700, MALDI-TOF mass spectrometry | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 45 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.423 | - |
Zymosterol | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Homo sapiens |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
tamoxifen | - |
Homo sapiens | |
| 0.001 | - |
tridemorph | - |
Homo sapiens | |
| 0.002 | - |
emopamil | - |
Homo sapiens | |
| 0.021 | - |
25-Azacholesterol | - |
Homo sapiens | |
| 0.156 | - |
ketoconazole | - |
Homo sapiens | |
| 0.62 | - |
cholesterol | 37°C | Homo sapiens | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | - |
- |
7.7 |
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