Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.3 extracted from

  • Scherf, U.; Buckel, W.
    Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA DELTA3-DELTA2-isomerase from Clostridium aminobutyricum (1993), Eur. J. Biochem., 215, 421-429.
    View publication on PubMed
Search for more literature for 5.3.3.3

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Clostridium aminobutyricum
additional information not inhibited by Fe2+, Mn2+, Co2+, Ni2+, Ca2+, Mg2+, Zn2+ Clostridium aminobutyricum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe-S 16 mol Fe and 14 mol S per mol of enzyme, iron-sulfur protein, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum
additional information no activation by Fe2+, Mn2+, Co2+, Ni2+, Ca2+, Mg2+, Zn2+ Clostridium aminobutyricum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium aminobutyricum
232000
-
 bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, gel filtration Clostridium aminobutyricum

Organism

Organism UniProt Comment Textmining
Clostridium aminobutyricum Q03161
-
-

Oxidation Stability

Oxidation Stability Organism
oxygen irreversibly inactivates Clostridium aminobutyricum

Purification (Commentary)

Purification (Comment) Organism
bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum

Subunits

Subunits Comment Organism
tetramer 4 * 56000, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity, SDS-PAGE Clostridium aminobutyricum

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of enzyme, bifunctional enzyme possessing 4-hydroxybutyryl-CoA dehydratase and vinylacetyl DELTA-isomerase activity Clostridium aminobutyricum