Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta cells | Manduca sexta |
expressed in Escherichia coli Rosetta cells | Choristoneura fumiferana |
Protein Variants | Comment | Organism |
---|---|---|
C113S | inactive | Choristoneura fumiferana |
C113S/114S | inactive | Choristoneura fumiferana |
C114S | inactive | Choristoneura fumiferana |
E169/171Q | inactive | Choristoneura fumiferana |
E169Q | inactive | Choristoneura fumiferana |
E171Q | inactive | Choristoneura fumiferana |
W216F | 10% activity compared to the wild type enzyme | Choristoneura fumiferana |
Y105F | 115% activity compared to the wild type enzyme | Choristoneura fumiferana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate | - |
Choristoneura fumiferana | |
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate | - |
Manduca sexta | |
(2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate | - |
Sus scrofa | |
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate | - |
Choristoneura fumiferana | |
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate | - |
Manduca sexta | |
(2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate | - |
Sus scrofa | |
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate | - |
Choristoneura fumiferana | |
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate | - |
Manduca sexta | |
(2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate | - |
Sus scrofa | |
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate | - |
Choristoneura fumiferana | |
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate | - |
Manduca sexta | |
2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate | - |
Sus scrofa | |
2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate | - |
Choristoneura fumiferana | |
2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate | - |
Manduca sexta | |
dimethylallyl diphosphate | - |
Choristoneura fumiferana | |
dimethylallyl diphosphate | - |
Manduca sexta | |
dimethylallyl diphosphate | - |
Sus scrofa | |
dimethylammonium diphosphate | - |
Choristoneura fumiferana | |
dimethylammonium diphosphate | - |
Manduca sexta | |
dimethylammonium diphosphate | - |
Sus scrofa | |
homodimethylallyl diphosphate | - |
Choristoneura fumiferana | |
homodimethylallyl diphosphate | - |
Manduca sexta | |
homodimethylallyl diphosphate | - |
Sus scrofa | |
homoisopentenyl diphosphate | - |
Choristoneura fumiferana | |
homoisopentenyl diphosphate | - |
Manduca sexta | |
homoisopentenyl diphosphate | - |
Sus scrofa | |
additional information | not inhibited by 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
isopentenyl diphosphate | wild type enzyme, in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | |
0.006 | - |
isopentenyl diphosphate | mutant enzyme E169Q, in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | |
0.006 | - |
isopentenyl diphosphate | mutant enzyme Y105F, in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | |
0.0116 | - |
isopentenyl diphosphate | mutant enzyme W216F, in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | |
0.0128 | - |
isopentenyl diphosphate | mutant enzyme Y159F, in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
mitochondrion | - |
Manduca sexta | 5739 | - |
mitochondrion | - |
Choristoneura fumiferana | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | preference for Mn2+ over Mg2+ | Sus scrofa | |
Mg2+ | preference for Mn2+ over Mg2+ | Manduca sexta | |
Mg2+ | preference for Mn2+ over Mg2+ | Choristoneura fumiferana | |
Mn2+ | preference for Mn2+ over Mg2+ | Sus scrofa | |
Mn2+ | preference for Mn2+ over Mg2+ | Manduca sexta | |
Mn2+ | preference for Mn2+ over Mg2+ | Choristoneura fumiferana | |
additional information | not activated by Zn2*, Fe2*, or Cu2+ | Choristoneura fumiferana | |
additional information | not activated by Zn2+, Fe2+, or Cu2+ | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29200 | - |
x * 29200, calculated from amino acid sequence | Manduca sexta |
29200 | - |
x * 29200, calculated from amino acid sequence | Choristoneura fumiferana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | Sus scrofa | - |
dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | Manduca sexta | - |
dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | Choristoneura fumiferana | - |
dimethylallyl diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Choristoneura fumiferana | - |
- |
- |
Manduca sexta | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography | Manduca sexta |
TALON metal affinity resin column chromatography | Choristoneura fumiferana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus allatum | highly abundant in the corpora allata | Manduca sexta | - |
liver | - |
Sus scrofa | - |
midgut | - |
Choristoneura fumiferana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isopentenyl diphosphate | - |
Sus scrofa | dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | - |
Manduca sexta | dimethylallyl diphosphate | - |
? | |
isopentenyl diphosphate | - |
Choristoneura fumiferana | dimethylallyl diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29200, calculated from amino acid sequence | Manduca sexta |
? | x * 29200, calculated from amino acid sequence | Choristoneura fumiferana |
Synonyms | Comment | Organism |
---|---|---|
IPPI | - |
Sus scrofa |
IPPI | - |
Manduca sexta |
IPPI | - |
Choristoneura fumiferana |
isopentenyl diphosphate isomerase | - |
Sus scrofa |
isopentenyl diphosphate isomerase | - |
Manduca sexta |
isopentenyl diphosphate isomerase | - |
Choristoneura fumiferana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Manduca sexta | calculated from amino acid sequence | - |
6 |
Choristoneura fumiferana | calculated from amino acid sequence | - |
6.4 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0008 | - |
in 50 mM Tris-HCl (pH 7.0), at 37°C | Sus scrofa | dimethylammonium diphosphate | |
0.003 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate | |
0.0055 | - |
in 50 mM Tris-HCl (pH 7.0), at 37°C | Sus scrofa | 2-[ethyl(methyl)amino]ethyl trihydrogen diphosphate | |
0.007 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | 2-[ethyl(propyl)amino]ethyl trihydrogen diphosphate | |
0.066 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | homoisopentenyl diphosphate | |
0.092 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | dimethylallyl diphosphate | |
0.131 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | homodimethylallyl diphosphate | |
0.156 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | (2Z)-3-methylhex-2-en-1-yl trihydrogen diphosphate | |
0.158 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | (2Z)-3-methylhept-2-en-1-yl trihydrogen diphosphate | |
0.234 | - |
in 25 mM Tris-HCl, pH 7.0, at 37°C | Choristoneura fumiferana | (2Z)-3-methyloct-2-en-1-yl trihydrogen diphosphate |