Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, ligand-free form of the FMN-bound enzyme form at 2.8 A resolution. The octamer forms a D4 symmetrical open, cage-like structure. The monomers of 45000 Da display a classical TIM barrel fold | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
8 * 45000 | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P50740 | type II isopentenyl diphosphate:dimethylallyl diphosphate isomerase | - |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 45000 | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
IDI | - |
Bacillus subtilis |
isopentenyl diphosphate:dimethylallyl diphosphate isomerase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | bound only with very moderate affinity and is therefore completely lost during purification. However, the enzyme can be reconstituted in the crystals by soaking with FMN | Bacillus subtilis |