Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.3.3.19 extracted from

  • Rajavel, M.; Mitra, A.; Gopal, B.
    Role of Bacillus subtilis BacB in the synthesis of bacilysin (2009), J. Biol. Chem., 284, 31882-31892.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.3.19

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis
expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
K107A significant decrease in catalytic activity when compared with the native enzyme Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.423
-
 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate pH 8.1, 37°C Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the protein is a bi-cupin, with two putative active sites each containing a bound metal ion Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis P39639
-
-
Bacillus subtilis 168 P39639
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
3-[(4R)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis 168 (3Z)-3-[(4R)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?
3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate
-
 Bacillus subtilis 168 (3E)-3-[(4S)-4-hydroxycyclohex-2-en-1-ylidene]-2-oxopropanoate
-
 ?

Synonyms

Synonyms Comment Organism
BacB
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Bacillus subtilis
37
-
 assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
24.52
-
 3-[(4S)-4-hydroxycyclohexa-1,5-dien-1-yl]-2-oxopropanoate pH 8.1, 37°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
 assay at Bacillus subtilis

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of the dipeptide antibiotic bacilysin Bacillus subtilis