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Literature summary for 5.3.3.17 extracted from
- The purification, crystallization and preliminary structural characterization of PhzF, a key enzyme in the phenazine-biosynthesis pathway from Pseudomonas fluorescens 2-79 (2004), Acta Crystallogr. Sect. D, 60, 184-186.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| large-scale expression in Escherichia coli | Pseudomonas fluorescens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapour-diffusion method. Crystallized from PEG 4000/ammonium sulfate/sodium citrate pH 5.6. The crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 56.3, c = 156.4 A. They contain one monomer in the asymmetric unit and diffract to better than 1.7 A on synchrotron beamlines. Crystals of seleno-L-methionine-labelled PhzF have been obtained | Pseudomonas fluorescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
two protein species with MW 32048 Da and 32224 Da, MALDI-TOF mass-spectrometry | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | Q51792 | strain 2-79 | - |
| Pseudomonas fluorescens Feb 79 | Q51792 | strain 2-79 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| phzF | - |
Pseudomonas fluorescens |
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