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Literature summary for 5.3.3.13 extracted from

  • Liavonchanka, A.; Rudolph, M.G.; Tittmann, K.; Hamberg, M.; Feussner, I.
    On the mechanism of a polyunsaturated fatty acid double bond isomerase from Propionibacterium acnes (2009), J. Biol. Chem., 284, 8005-8012.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into the vector pGEX-6P-1 for expression in Escherichia coli cells Cutibacterium acnes

Protein Variants

Protein Variants Comment Organism
F168G/G169F inactive mutant Cutibacterium acnes
F168L inactive mutant Cutibacterium acnes
F193A mutant Cutibacterium acnes
R88A inactive mutant Cutibacterium acnes
R88S mutant Cutibacterium acnes
R88S/F193A mutant Cutibacterium acnes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information (11S-2H) linoleic acid, 0.00408, wild-type PAI Cutibacterium acnes
additional information
-
additional information [11,11-2H2] linoleic acid, 0.00228, wild-type PAI Cutibacterium acnes
0.00482
-
linoleic acid wild-type PAI Cutibacterium acnes
0.02577
-
linoleic acid mutant R88S/F193A Cutibacterium acnes
0.03794
-
linoleic acid mutant F193A Cutibacterium acnes
0.0657
-
linoleic acid mutant R88S Cutibacterium acnes

Organism

Organism UniProt Comment Textmining
Cutibacterium acnes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cutibacterium acnes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid
-
Cutibacterium acnes ?
-
?
linoleic acid methyl ester
-
Cutibacterium acnes (10E,12Z)-octadeca-10,12-dienoic acid methyl ester
-
?
linoleoyl-CoA
-
Cutibacterium acnes (10Z,12Z)-octadeca-10,12-dienoyl-CoA
-
?

Synonyms

Synonyms Comment Organism
PAI
-
Cutibacterium acnes
polyunsaturated fatty acid double bond isomerase
-
Cutibacterium acnes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
kinetic assay Cutibacterium acnes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information (11S-2H) linoleic acid, 3.51, wild-type PAI Cutibacterium acnes
additional information
-
additional information [11,11-2H2] linoleic acid, 0.83, wild-type PAI Cutibacterium acnes
0.16
-
linoleic acid mutant R88S/F193A Cutibacterium acnes
0.33
-
linoleic acid mutant F193A Cutibacterium acnes
2.93
-
linoleic acid mutant R88S Cutibacterium acnes
4.37
-
linoleic acid wild-type PAI Cutibacterium acnes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
activity and kinetic assay Cutibacterium acnes

Cofactor

Cofactor Comment Organism Structure
FAD
-
Cutibacterium acnes