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Literature summary for 5.3.3.12 extracted from

  • Vavricka, C.J.; Ray, K.W.; Christensen, B.M.; Li, J.
    Purification and N-glycosylation analysis of melanoma antigen dopachrome tautomerase (2010), Protein J., 29, 204-212.
    View publication on PubMed
Search for more literature for 5.3.3.12

Application

Application Comment Organism
medicine DCT is processed as a melanoma antigen and is a potential target for immunotherapy Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the truncated enzyme lacking its carboxy-terminal transmembrane region in Spodoptera frugiperda Sf9 insect cell using a viral transfer vector Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane DCT has a hydrophobic transmembrane anchor Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-dopachrome Homo sapiens
-
 5,6-dihydroxyindole-2-carboxylate
-
 ?
additional information Homo sapiens DCT interacts with melanin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein Analysis of DCT tryptic peptides by MALDI-TOF/TOF determines N-glycosylation as a primary post-translational modification Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymne from Sf9 insect cells by ammonium sulfate fractionation, hydroxyapatite and anion exchange chromatography, and gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
melanoma cell levels of DCT are elevated in melanoma cell lines that are especially resistant to chemotherapy and radiation Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dopachrome
-
 Homo sapiens 5,6-dihydroxyindole-2-carboxylate
-
 ?
additional information structure-function relationship, overview Homo sapiens ?
-
 ?
additional information DCT interacts with melanin Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More structure-function relationship, overview Homo sapiens

Synonyms

Synonyms Comment Organism
DCT
-
 Homo sapiens
dopachrome tautomerase
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function DCT plays a critical role in lowering the oxidative stress resulting from melanogenesis Homo sapiens