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Literature summary for 5.3.3.1 extracted from

  • Yun, Y.S.; Nam, G.H.; Kim, Y.G.; Oh, B.H.; Choi, K.Y.
    Small exterior hydrophobic cluster contributes to conformational stability and steroid binding in ketosteroid isomerase from Pseudomonas putida biotype B (2005), FEBS J., 272, 1999-2011.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
mutant W92A, in complex with d-equilenin, decrease in conformational stability results from destabilization of surface hydrophobic layer Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
L125A/V127A mutation in small exterior hydrophobic cluster, decrease in conformational stability Pseudomonas putida
L125F/V127F mutation in small exterior hydrophobic cluster, slight increase in stability Pseudomonas putida
W92A mutation in small exterior hydrophobic cluster, decrease in conformational stability. Crystallization data Pseudomonas putida
W92A/L125A/V127A mutation in small exterior hydrophobic cluster, decrease in conformational stability Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0499
-
 5-androstene-3,17-dione wild-type, 25°C, pH 7.0 Pseudomonas putida
0.0795
-
 5-androstene-3,17-dione mutant L125F/V127F, 25°C, pH 7.0 Pseudomonas putida
0.0995
-
 5-androstene-3,17-dione mutant W92A, 25°C, pH 7.0 Pseudomonas putida
0.1185
-
 5-androstene-3,17-dione mutant W92A/L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
0.1375
-
 5-androstene-3,17-dione mutant L125A/V127A, 25°C, pH 7.0 Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
 biotype B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-Androstene-3,17-dione
-
 Pseudomonas putida 4-Androstene-3,17-dione
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12000
-
 5-androstene-3,17-dione mutant W92A/L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
12700
-
 5-androstene-3,17-dione mutant W92A, 25°C, pH 7.0 Pseudomonas putida
12900
-
 5-androstene-3,17-dione mutant L125A/V127A, 25°C, pH 7.0 Pseudomonas putida
17600
-
 5-androstene-3,17-dione mutant L125F/V127F, 25°C, pH 7.0 Pseudomonas putida
21200
-
 5-androstene-3,17-dione wild-type, 25°C, pH 7.0 Pseudomonas putida