Crystallization (Comment) | Organism |
---|---|
mutant enzyme D99E/D38N complexed with equilenin, an intermediate analogue, crystals of the complexes are grown from 1.1 M ammonium acetate and 0.1 M sodium acetate, pH 4.6, by the hanging drop vapor diffusion method at 22°C. The resulting crystals have C2 space group symmetry with unit cell dimensions of a = 89.04 A, b = 72.42 A, c = 51.24 A and beta = 90.9° | Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
D99E | turnover-number for 5-androstene-3,17-dione is 68.1% of the turnover number of the wild-type enzyme, the Km-value is 1.22fold higher than the Km-value of the wild-type enzyme | Pseudomonas putida |
D99L | turnover-number for 5-androstene-3,17-dione is 125fold lower than the turnover number of the wild-type enzyme, the Km-value is 1.95fold lower than the Km-value of the wild-type enzyme | Pseudomonas putida |
Y14F | turnover-number for 5-androstene-3,17-dione is less than 1% of the turnover number of the wild-type enzyme, the Km-value is 1.87fold lower than the Km-value of the wild-type enzyme | Pseudomonas putida |
Y14F/D99E | turnover-number for 5-androstene-3,17-dione is less than 1% of the turnover number of the wild-type enzyme, the Km-value is 1.05fold lower than the Km-value of the wild-type enzyme | Pseudomonas putida |
Y14F/D99L | turnover-number for 5-androstene-3,17-dione is less than 1% of the turnover number of the wild-type enzyme, the Km-value is 1.95fold higher than the Km-value of the wild-type enzyme | Pseudomonas putida |
Y14F/D99N | turnover-number for 5-androstene-3,17-dione is less than 1% of the turnover number of the wild-type enzyme, the Km-value is 1.06fold lower than the Km-value of the wild-type enzyme | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
19-nortestosterone | - |
Pseudomonas putida | |
equilenin | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0269 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F | Pseudomonas putida | |
0.0475 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99N | Pseudomonas putida | |
0.0481 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99E | Pseudomonas putida | |
0.0503 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, wild-type enzyme | Pseudomonas putida | |
0.0616 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme D99E | Pseudomonas putida | |
0.0982 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99L | Pseudomonas putida | |
0.258 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme D99L | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-Androstene-3,17-dione | - |
Pseudomonas putida | 4-Androstene-3,17-dione | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.67 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99L | Pseudomonas putida | |
0.7 | 1 | 5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99N | Pseudomonas putida | |
0.71 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99N | Pseudomonas putida | |
3 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99E | Pseudomonas putida | |
6.08 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F/D99L | Pseudomonas putida | |
11.5 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme Y14F | Pseudomonas putida | |
220 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme D99L | Pseudomonas putida | |
19000 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, mutant enzyme D99E | Pseudomonas putida | |
27900 | - |
5-androstene-3,17-dione | pH 7.0, 25°C, wild-type enzyme | Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki-values for equilenin and 19-nortestosterone, wild-type enzyme and mutant enzyme D99E | Pseudomonas putida |