Protein Variants | Comment | Organism |
---|---|---|
Y55F/Y88F | replacement of Tyr14 by 3-fluorotyrosine in the Y55,88F modified form of the isomerase results in a 4-fold decrease in turnover number | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 3-oxo-DELTA5-steroid = a 3-oxo-DELTA4-steroid | mechanism in which the transition state for enolization is dienolate-like, characterized by relatively little proton transfer from Tyr14 in the transition state, and the intermediate in the overall reaction is dienol-like. An alternative mechanism in which the intermediate is stabilized by a short, strong hydrogen bond can also be consistent with the data | Comamonas testosteroni |