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Literature summary for 5.3.2.6 extracted from

  • Fitzgerald, M.; Chernushevich, I.; Standing, K.; Kent, S.; Whitman, C.
    Total chemical synthesis and catalytic properties of the enzyme enantiomers L- and D-4-oxalocrotonate tautomerase (1995), J. Am. Chem. Soc., 117, 11075-11080.
No PubMed abstract available
Search for more literature for 5.3.2.6

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida
0.103
-
 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxo-4(E)-hexenedioate Pseudomonas putida 1,3-allylic isomerization 2-oxo-3(E)-hexenedioate
-
 ?
2-oxo-4(E)-hexenedioate Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 1,3-allylic isomerization 2-oxo-3(E)-hexenedioate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4(E)-hexenedioate 1,3-allylic isomerization Pseudomonas putida 2-oxo-3(E)-hexenedioate
-
 ?
2-oxo-4(E)-hexenedioate 1,3-allylic isomerization Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 2-oxo-3(E)-hexenedioate
-
 ?
additional information the achiral substrate 2-hydroxymuconate is processed with equal efficiency by either the D- or the L-enzyme, stereochemical analysis of the D-4OT-catalyzed reaction, overviewn Pseudomonas putida ?
-
 ?
additional information the achiral substrate 2-hydroxymuconate is processed with equal efficiency by either the D- or the L-enzyme, stereochemical analysis of the D-4OT-catalyzed reaction, overviewn Pseudomonas putida mt-2 / ATCC 33015 / DSM 3931 / NCIB 12182 / NCIMB 12182 ?
-
 ?

Subunits

Subunits Comment Organism
hexamer the noncovalent structures of each 4OT enantiomer are similar, the predominant form of the enzyme is a hexameric complex of identical subunits Pseudomonas putida

Synonyms

Synonyms Comment Organism
4-oxalocrotonate tautomerase
-
 Pseudomonas putida
4OT
-
 Pseudomonas putida
D-4-oxalocrotonate tautomerase
-
 Pseudomonas putida
L-4-oxalocrotonate tautomerase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Pseudomonas putida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2890
-
 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida
2940
-
 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Pseudomonas putida

General Information

General Information Comment Organism
metabolism 4-oxalocrotonate tautomerase is part of a set of inducible enzymes that converts aromatic hydrocarbons to intermediates in the Krebs cycle Pseudomonas putida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
29000
-
 2-oxo-4(E)-hexenedioate L-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida
32000
-
 2-oxo-4(E)-hexenedioate D-4OT enantiomer, pH 7.3, 30°C Pseudomonas putida