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Literature summary for 5.3.2.6 extracted from

  • Azurmendi, H.F.; Miller, S.G.; Whitman, C.P.; Mildvan, A.S.
    Half-of-the-sites binding of reactive intermediates and their analogues to 4-oxalocrotonate tautomerase and induced structural asymmetry of the enzyme (2005), Biochemistry, 44, 7725-7737.
    View publication on PubMed
Search for more literature for 5.3.2.6

Protein Variants

Protein Variants Comment Organism
R39Q titration with cis,cis-muconate shows negative cooperativity Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
(2E)-2-fluoro-2,4-pentadienoate
-
 Pseudomonas putida
(2E)-fluoromuconate a dicarboxylate competitive inhibitor, shows substoichiometric binding to 3 +/- 1 sites per hexamer Pseudomonas putida
(2Z)-2-fluoro-2,4-pentadienoate
-
 Pseudomonas putida
additional information although 4-OT exhibits no structural asymmetry either by X-ray or by NMR, inactivation by two affinity labels shows half-site stoichiometry Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4-OT exhibits hyperbolic kinetics and shows anticooperativity during catalysis Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-4-hexenedioate the enzyme converts the unconjugated enone to the conjugated enone via a dienolic intermediate 2-hydroxymuconate, Pro1 serves as the general base, and both Arg11 and Arg39 function in substrate binding and catalysis in an otherwise hydrophobic active site. Anticooperativity during catalysis Pseudomonas putida 2-oxo-3-hexenedioate
-
 r

Subunits

Subunits Comment Organism
homohexamer
-
 Pseudomonas putida

Synonyms

Synonyms Comment Organism
4-OT
-
 Pseudomonas putida
4-oxalocrotonate tautomerase
-
 Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.045
-
 (2E)-fluoromuconate pH and temperature not specified in the publication Pseudomonas putida

General Information

General Information Comment Organism
additional information structure-function relationship and kinetic analysis, detailed overview Pseudomonas putida