Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Aneurinibacillus thermoaerophilus |
General Stability | Organism |
---|---|
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol | Aneurinibacillus thermoaerophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | Aneurinibacillus thermoaerophilus | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aneurinibacillus thermoaerophilus | Q6T1W8 | - |
- |
Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | Q6T1W8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Aneurinibacillus thermoaerophilus |
Storage Stability | Organism |
---|---|
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity | Aneurinibacillus thermoaerophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | Aneurinibacillus thermoaerophilus | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis | Aneurinibacillus thermoaerophilus | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The glycan chain of the S-layer protein of Aneurinibacillus thermoaerophilus L420-91T (DSM 10154) consists of D-rhamnose and 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc). Thymidine diphosphate-activated (D-Fucp3NAc) serves as precursor for the assembly of structural polysaccharides in Gram-positive and Gram-negative organisms. The biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose (dTDP-D-Fucp3NAc) involves five enzymes. FdtA is involved in this pathway | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r | |
dTDP-4-dehydro-6-deoxy-alpha-D-glucopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-4-ulose. The activity of FdtA is monitored by a simultaneous incubation with dTDP-6-deoxy-D-xylohex-4-ulose with FdtB and the required cosubstrates. The resulting product, dTDP-D-Fucp3N, is detectable by HPLC analysis | Aneurinibacillus thermoaerophilus L420-91T, DSM 10154 | dTDP-3-dehydro-6-deoxy-alpha-D-galactopyranose | i.e. dTDP-6-deoxy-D-xylo-hexopyranos-3-ulose | r |
Synonyms | Comment | Organism |
---|---|---|
FdtA | - |
Aneurinibacillus thermoaerophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aneurinibacillus thermoaerophilus |
50 | 55 | - |
Aneurinibacillus thermoaerophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Aneurinibacillus thermoaerophilus |