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BRENDA support

Literature summary for 5.3.2.1 extracted from

  • Cherepkova, O.A.; Lyutova, E.M.; Gurvits, B.Y.
    Macrophage migration inhibitory factor: isolation from bovine brain (2006), Biochemistry (Moscow), 71, 73-78.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Bos taurus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
x * 12000, SDS-PAGE Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
macrophage migration inhibitory factor
-

Purification (Commentary)

Purification (Comment) Organism
simple method for purification based on size-exclusion chromatography on Toyopearl TSK polymer having a tendencey to adsorb the enzyme Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, protein from homogenated tissue heated to 58°C for 20 min, and subsequently salted out of the supernatant with ammonium sulfate to the final saturation of 80% can be stored six months without loss of activity Bos taurus

Subunits

Subunits Comment Organism
? x * 12000, SDS-PAGE Bos taurus