Protein Variants | Comment | Organism |
---|---|---|
E52Q | mutation in alpha-subunit, 1.6fold increase in ratio of kcat to Km value | Pseudomonas pavonaceae |
P1A | mutation in beta-subunit, 8fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
R11A | mutation in alpha-subunit, 15fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
R8A | mutation in alpha-subunit, 19fold decrease in ratio of kcat to Km value | Pseudomonas pavonaceae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
enol-phenylpyruvate | pH 9.0 | Mus musculus | |
0.031 | - |
enol-phenylpyruvate | pH 6.8 | Mus musculus | |
0.061 | - |
enol-phenylpyruvate | wild-type, pH 9.0 | Pseudomonas pavonaceae | |
0.113 | - |
enol-phenylpyruvate | mutant E52Q, pH 9.0 | Pseudomonas pavonaceae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
macrophage migration inhibitory factor MIF | - |
Pseudomonas pavonaceae | - |
trans-3-chloroacrylic acid dehydrogenase CaaD has phenylpyruvate tautomerase activity | - |
Pseudomonas pavonaceae 170 | - |
trans-3-chloroacrylic acid dehydrogenase CaaD has phenylpyruvate tautomerase activity | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
enol-phenylpyruvate | - |
Mus musculus | keto-phenylpyruvate | reaction is stereoselective, generating the (3R) isomer of [3-D]phenylpyruvate in D2O in a ratio of 6.6:1 | ? | |
enol-phenylpyruvate | reaction is stereoselective, generating the 3S isomer of [3-D]phenylpyruvate in a 18:1 ratio in D2O | Pseudomonas pavonaceae | keto-phenylpyruvate | - |
? | |
enol-phenylpyruvate | reaction is stereoselective, generating the 3S isomer of [3-D]phenylpyruvate in a 18:1 ratio in D2O | Pseudomonas pavonaceae 170 | keto-phenylpyruvate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CaaD | - |
Pseudomonas pavonaceae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
enol-phenylpyruvate | wild-type, pH 9.0 | Pseudomonas pavonaceae | |
4.1 | - |
enol-phenylpyruvate | mutant E52Q, pH 9.0 | Pseudomonas pavonaceae | |
33 | - |
enol-phenylpyruvate | pH 9.0 | Mus musculus | |
38 | - |
enol-phenylpyruvate | pH 6.8 | Mus musculus |