Cloned (Comment) | Organism |
---|---|
GPI gene, DNA and amino acid sequence determination and analysis, phylogenetic tree and expression analysis by real time quantitative RT-PCR, recombinant expression of His-tagged enzyme in Escherichia coli | Dunaliella salina |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73300 | - |
x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation | Dunaliella salina |
75000 | - |
x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation | Dunaliella salina |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Dunaliella salina | B6E5W6 | UTEX-LB-1644 | - |
Dunaliella salina UTEX-LB-1644 | B6E5W6 | UTEX-LB-1644 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Dunaliella salina |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation | Dunaliella salina |
More | predicted 2D and 3D structures of GPI proteins possess potential active motifs including GEPGTNGQHSFYQLIHQG and VQGFIWGINSFDQWGVELGK, and critical active site residues, such as Ser241, Ser296, Thr298, Thr301, Arg358, Glu444, His475 and Lys600 | Dunaliella salina |
Synonyms | Comment | Organism |
---|---|---|
GPI | - |
Dunaliella salina |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Dunaliella salina | sequence calculation | - |
6.22 |
General Information | Comment | Organism |
---|---|---|
metabolism | GPI plays an important role in glycolysis | Dunaliella salina |