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Literature summary for 5.3.1.9 extracted from

  • Cui, L.; Xue, L.; Li, J.; Zhang, L.; Yan, H.
    Characterization of the glucose-6-phosphate isomerase (GPI) gene from the halotolerant alga Dunaliella salina (2010), Mol. Biol. Rep., 37, 911-916.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
GPI gene, DNA and amino acid sequence determination and analysis, phylogenetic tree and expression analysis by real time quantitative RT-PCR, recombinant expression of His-tagged enzyme in Escherichia coli Dunaliella salina

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73300
-
x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation Dunaliella salina
75000
-
x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation Dunaliella salina

Organism

Organism UniProt Comment Textmining
Dunaliella salina B6E5W6 UTEX-LB-1644
-
Dunaliella salina UTEX-LB-1644 B6E5W6 UTEX-LB-1644
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Dunaliella salina

Subunits

Subunits Comment Organism
? x * 75000, recombinant His-tagged enzyme, SDS-PAGE, x * 73300, about, sequence calculation Dunaliella salina
More predicted 2D and 3D structures of GPI proteins possess potential active motifs including GEPGTNGQHSFYQLIHQG and VQGFIWGINSFDQWGVELGK, and critical active site residues, such as Ser241, Ser296, Thr298, Thr301, Arg358, Glu444, His475 and Lys600 Dunaliella salina

Synonyms

Synonyms Comment Organism
GPI
-
Dunaliella salina

pI Value

Organism Comment pI Value Maximum pI Value
Dunaliella salina sequence calculation
-
6.22

General Information

General Information Comment Organism
metabolism GPI plays an important role in glycolysis Dunaliella salina