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Literature summary for 5.3.1.7 extracted from
- Growth on D-lyxose of a mutant strain of Escherichia coli K12 using a novel isomerase and enzymes related to D-xylose metabolism (1976), J. Gen. Microbiol., 97, 257-265.
General Stability
| General Stability | Organism |
|---|---|
| stable in presence of 1 mM 2-mercaptoethanol or EDTA | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Escherichia coli |  |
| Cu2+ | - |
Escherichia coli | |
| GSH | 5 mM | Escherichia coli | |
| Hg2+ | - |
Escherichia coli | |
| KCl | 0.2 M | Escherichia coli | |
| NaCl | 0.2 M | Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 80 | - |
D-mannose | - |
Escherichia coli | |
| 300 | - |
D-Lyxose | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 160000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Mannose | Escherichia coli | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidovorax avenae | - |
- |
- |
| Acidovorax avenae S-48 | - |
- |
- |
| Escherichia coli | - |
K12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Lyxose | - |
Escherichia coli | D-Xylulose | - |
? | |
| D-Mannose | - |
Escherichia coli | D-Fructose | - |
? | |
| D-Mannose | - |
Acidovorax avenae | D-Fructose | - |
? | |
| D-Mannose | - |
Acidovorax avenae S-48 | D-Fructose | - |
? | |
| D-Mannose | - |
Escherichia coli | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.2 | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | pH 6: about 35% of maximal activity, pH 9.5: about 40% of maximal activity | Escherichia coli |
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