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Literature summary for 5.3.1.6 extracted from
- Substrate specificity of a recombinant ribose-5-phosphate isomerase from Streptococcus pneumoniae and its application in the production of l-lyxose and l-tagatose (2011), World J. Microbiol. Biotechnol., 27, 743-750.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | RpiB is a potential producer of L-form monosaccharides | Streptococcus pneumoniae |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli ER2566 cells | Streptococcus pneumoniae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not activated by monovalent or divalent cations | Streptococcus pneumoniae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23724 | - |
4 * 23724, calculated from amino acid sequence | Streptococcus pneumoniae |
| 24000 | - |
4 * 24000, SDS-PAGE | Streptococcus pneumoniae |
| 96000 | - |
gel filtration | Streptococcus pneumoniae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribose 5-phosphate | Streptococcus pneumoniae | highest specific activity | D-ribulose 5-phosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap Q anion exchange column chromatography and Sephacryl S-300 gel filtration | Streptococcus pneumoniae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0005 | - |
using D-sorbose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0011 | - |
using L-fructose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0013 | - |
using L-tagatose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0019 | - |
using D-allose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0021 | - |
using D-psicose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0025 | - |
using D-ribose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0053 | - |
using D-gulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.0096 | - |
using L-lyxose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.019 | - |
using L-talose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.027 | - |
using L-lyxose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.048 | - |
using D-ribulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.058 | - |
using D-glucose 6-phosphate as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.121 | - |
using L-xylulose as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
| 0.388 | - |
using D-ribose 5-phosphate as substrate, at 35°C and pH 7.5 | Streptococcus pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-allose | - |
Streptococcus pneumoniae | D-psicose | - |
r | |
| D-Glucose 6-phosphate | - |
Streptococcus pneumoniae | ? | - |
? | |
| D-gulose | - |
Streptococcus pneumoniae | D-sorbose | - |
r | |
| D-psicose | - |
Streptococcus pneumoniae | D-allose | - |
r | |
| D-ribose | - |
Streptococcus pneumoniae | D-ribulose | - |
r | |
| D-ribose 5-phosphate | highest specific activity | Streptococcus pneumoniae | D-ribulose 5-phosphate | - |
? | |
| D-ribulose | - |
Streptococcus pneumoniae | D-ribose | - |
r | |
| D-sorbose | lowest activity with L-sorbose | Streptococcus pneumoniae | D-gulose | - |
r | |
| L-fructose | - |
Streptococcus pneumoniae | L-mannose | - |
r | |
| L-lyxose | particularly high activity with L-lyxose | Streptococcus pneumoniae | L-xylulose | - |
r | |
| L-Mannose | - |
Streptococcus pneumoniae | L-Fructose | - |
r | |
| L-tagatose | particularly high activity with L-tagatose | Streptococcus pneumoniae | L-talose | - |
r | |
| L-talose | particularly high activity with L-talose | Streptococcus pneumoniae | L-tagatose | - |
r | |
| L-xylulose | particularly high activity with L-lyxose | Streptococcus pneumoniae | L-lyxose | - |
r | |
| additional information | no activity is observed with D-mannose-6-phosphate | Streptococcus pneumoniae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 24000, SDS-PAGE | Streptococcus pneumoniae |
| homotetramer | 4 * 23724, calculated from amino acid sequence | Streptococcus pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ribose-5-phosphate isomerase | - |
Streptococcus pneumoniae |
| RpiB | - |
Streptococcus pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptococcus pneumoniae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | - |
Streptococcus pneumoniae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | enzyme activity is stable for 48 h at 35°C, and shows half-lives of 15 h at 40°C, and 6 h at 50°C | Streptococcus pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptococcus pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | - |
Streptococcus pneumoniae |
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