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Literature summary for 5.3.1.6 extracted from

  • MacElroy, R.D.; Middaugh, C.R.
    Bacterial ribosephosphate isomerase (1982), Methods Enzymol., 89, 571-579.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol stimulates Thiobacillus thioparus
2-mercaptoethanol stimulates [Bacillus] caldolyticus
EDTA stimulates Thiobacillus thioparus
EDTA stimulates [Bacillus] caldolyticus

General Stability

General Stability Organism
freeze-labile Thiobacillus thioparus

Inhibitors

Inhibitors Comment Organism Structure
6-phosphogluconate
-
[Bacillus] caldolyticus
6-phosphogluconate
-
Escherichia coli
6-phosphogluconate
-
Thiobacillus thioparus
AMP
-
[Bacillus] caldolyticus
AMP
-
Escherichia coli
AMP 10 mM Rhodospirillum rubrum
AMP
-
Thiobacillus thioparus
citrate 10 mM Rhodospirillum rubrum
fructose 6-phosphate
-
Escherichia coli
NAD+ 10 mM Rhodospirillum rubrum
ribulose diphosphate 1.0 mM, strong Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
gel filtration Thiobacillus thioparus
40000
-
gel filtration [Bacillus] caldolyticus
54000
-
-
Chromatium sp.
57000
-
-
Rhodospirillum rubrum

Organism

Organism UniProt Comment Textmining
Chromatium sp.
-
-
-
Chromatium sp. D
-
-
-
Escherichia coli
-
-
-
Halothiobacillus neapolitanus
-
-
-
Rhodospirillum rubrum
-
-
-
Thiobacillus thioparus
-
-
-
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.7
-
-
[Bacillus] caldolyticus
544
-
-
Thiobacillus thioparus

Storage Stability

Storage Stability Organism
4°C, stable for 2 weeks [Bacillus] caldolyticus
4°C, stable for 6-8 weeks Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribose 5-phosphate
-
Escherichia coli D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
Thiobacillus thioparus D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
[Bacillus] caldolyticus D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
Rhodospirillum rubrum D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
Chromatium sp. D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
Halothiobacillus neapolitanus D-ribulose 5-phosphate
-
?
D-ribose 5-phosphate
-
Chromatium sp. D D-ribulose 5-phosphate
-
?