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Literature summary for 5.3.1.4 extracted from

  • Wanarska, M.; Kur, J.
    A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting beta-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c (2012), Microb. Cell Fact., 11, 113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris and in Escherichia coli strain BL21(DE3)pLysS Arthrobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 82% residual activity at 10 mM Arthrobacter sp.
additional information no activity loss is observed after treatment with EDTA for 12 h Arthrobacter sp.
Ni2+ 70% residual activity at 10 mM Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
119
-
D-galactose at pH 7.0 and 50°C Arthrobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not dependent on divalent metal ions, since it is only marginally activated by Mg2+, Mn2+ or Ca2+ Arthrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
6 * 55000, SDS-PAGE Arthrobacter sp.
55183
-
6 * 55183, calculated from amino acid sequence Arthrobacter sp.
335000
-
gel filtration Arthrobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Arthrobacter sp.
-
L-ribulose
-
?
L-arabinose Arthrobacter sp. 22c
-
L-ribulose
-
?

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. H6UGW9
-
-
Arthrobacter sp. 22c H6UGW9
-
-

Purification (Commentary)

Purification (Comment) Organism
Fractogel EMD DEAE chromatography and Fractogel EMD TMAE chromatography Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.026
-
cell extract, at pH 7.5 and 52°C Arthrobacter sp.
0.232
-
after 8.9fold purification, at pH 7.5 and 52°C Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galactose
-
Arthrobacter sp. D-tagatose 30% yield after 36 h at 50°C ?
D-galactose
-
Arthrobacter sp. 22c D-tagatose 30% yield after 36 h at 50°C ?
L-arabinose
-
Arthrobacter sp. L-ribulose
-
?
L-arabinose
-
Arthrobacter sp. 22c L-ribulose
-
?

Subunits

Subunits Comment Organism
homohexamer 6 * 55000, SDS-PAGE Arthrobacter sp.
homohexamer 6 * 55183, calculated from amino acid sequence Arthrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
the enzyme exhibits over 60% of maximum activity at 30°C Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 70 over 90% of the initial activity is retained after 72 h incubation at 50°C. At 60 and 65°C, over 50% of initial activity is lost in incubations of 60 and 30 min, respectively. The enzyme is completely inactivated by 15 min at 70°C Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 the enzyme maintains over 90% of maximum activity in a pH range of 5.0 to 9.0 Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
over 90% of the initial activity is retained after 72 h incubation at pH 7.0 Arthrobacter sp.

pI Value

Organism Comment pI Value Maximum pI Value
Arthrobacter sp. calculated from amino acid sequence
-
5.1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0023
-
D-galactose at pH 7.0 and 50°C Arthrobacter sp.