Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Parageobacillus thermoglucosidasius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong inhibition at 1 mM | Parageobacillus thermoglucosidasius | |
Cu2+ | complete at 1 mM | Parageobacillus thermoglucosidasius | |
Fe2+ | strong inhibition at 1 mM | Parageobacillus thermoglucosidasius | |
Zn2+ | strong inhibition at 1 mM | Parageobacillus thermoglucosidasius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
175 | - |
D-galactose | at pH 7.0 and 40°C | Parageobacillus thermoglucosidasius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates 2.5fold at 1 mM | Parageobacillus thermoglucosidasius | |
additional information | not influenced by Mg2+ and Ca2+ | Parageobacillus thermoglucosidasius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, calculated from amino acid sequence | Parageobacillus thermoglucosidasius |
72000 | - |
x * 72000, N-terminal fusion-tagged enzyme, SDS-PAGE | Parageobacillus thermoglucosidasius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arabinose | Parageobacillus thermoglucosidasius | - |
L-ribulose | - |
? | |
L-arabinose | Parageobacillus thermoglucosidasius KCTC 1828 | - |
L-ribulose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Parageobacillus thermoglucosidasius | Q6VAK6 | - |
- |
Parageobacillus thermoglucosidasius KCTC 1828 | Q6VAK6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Parageobacillus thermoglucosidasius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galactose | - |
Parageobacillus thermoglucosidasius | D-tagatose | 45.6% conversion at 40°C | ? | |
D-galactose | - |
Parageobacillus thermoglucosidasius KCTC 1828 | D-tagatose | 45.6% conversion at 40°C | ? | |
L-arabinose | - |
Parageobacillus thermoglucosidasius | L-ribulose | - |
? | |
L-arabinose | - |
Parageobacillus thermoglucosidasius KCTC 1828 | L-ribulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, calculated from amino acid sequence | Parageobacillus thermoglucosidasius |
? | x * 72000, N-terminal fusion-tagged enzyme, SDS-PAGE | Parageobacillus thermoglucosidasius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Parageobacillus thermoglucosidasius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | more than 80% activity between 35 and 45°C | Parageobacillus thermoglucosidasius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | the enzyme retains more than 80% activity after 2 h when incubated at 30, 40, 50, or 60°C | Parageobacillus thermoglucosidasius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Parageobacillus thermoglucosidasius |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 30% activity at pH 6.0 and 9.0, 100% activity at pH 7.0, 90% activity at pH 8.0 | Parageobacillus thermoglucosidasius |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
D-galactose | at pH 7.0 and 40°C | Parageobacillus thermoglucosidasius |