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Literature summary for 5.3.1.4 extracted from

  • Lee, S.J.; Lee, S.J.; Lee, Y.J.; Kim, S.B.; Kim, S.K.; Lee, D.W.
    Homologous alkalophilic and acidophilic L-arabinose isomerases reveal region-specific contributions to the pH dependence of activity and stability (2012), Appl. Environ. Microbiol., 78, 8813-8816.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.3.1.4

Protein Variants

Protein Variants Comment Organism
M33R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
Q202R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
R200E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
R216E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
R31E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
R48E the mutant has the pH optimum of stability shifted downward from basic to acidic pH Alicyclobacillus sp.
V50R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius
Y218R the mutant has the pH optimum of stability shifted upward from acidic to basic pH Alicyclobacillus acidocaldarius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arabinose Alicyclobacillus acidocaldarius
-
 L-ribulose
-
 ?
L-arabinose Alicyclobacillus sp.
-
 L-ribulose
-
 ?
L-arabinose Alicyclobacillus sp. TP7
-
 L-ribulose
-
 ?

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
-
-
Alicyclobacillus sp.
-
-
-
Alicyclobacillus sp. TP7
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arabinose
-
 Alicyclobacillus acidocaldarius L-ribulose
-
 ?
L-arabinose
-
 Alicyclobacillus sp. L-ribulose
-
 ?
L-arabinose
-
 Alicyclobacillus sp. TP7 L-ribulose
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Alicyclobacillus acidocaldarius
8.5
-
-
 Alicyclobacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9 the wild type enzyme shows half-lifes of activity of 14, 90 and 64.4 h at pH 5.0, 7.0, and 9.0, respectively Alicyclobacillus acidocaldarius
5 9 the wild type enzyme shows half-lifes of activity of 6.9, 201 and 334 h at pH 5.0, 7.0, and 9.0, respectively Alicyclobacillus sp.