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Literature summary for 5.3.1.4 extracted from
- Bifidobacterium longum L-arabinose isomerase - overexpression in Lactococcus lactis, purification, and characterization (2012), Appl. Biochem. Biotechnol., 168, 392-405.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Lactococcus lactis | Bifidobacterium longum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | moderate inhibitory effect at 1 mM | Bifidobacterium longum |  |
| EDTA | 30% inhibition at 1 mM | Bifidobacterium longum | |
| Fe2+ | strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe2+ | Bifidobacterium longum | |
| Fe3+ | strong inhibitory effect, over 70% of the activity is lost already at 0.5 mM Fe3+ | Bifidobacterium longum | |
| Mn2+ | moderate inhibitory effect at 1 mM | Bifidobacterium longum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 120 | - |
L-arabinose | at pH 6.5 and 45°C | Bifidobacterium longum | |
| 590 | - |
D-galactose | at pH 6.5 and 45°C | Bifidobacterium longum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | the addition of Ca2+ has slightly positive but no significant effect on the enzyme activity | Bifidobacterium longum | |
| Mg2+ | the addition of Mg2+ has slightly positive but no significant effect on the enzyme activity | Bifidobacterium longum | |
| additional information | the enzyme has very low requirement for metal ions for catalytic activity, but it is stabilized by divalent metal ions (Mg2+, Mn2+) | Bifidobacterium longum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, SDS-PAGE | Bifidobacterium longum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-arabinose | Bifidobacterium longum | - |
L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
| L-arabinose | Bifidobacterium longum NRRL B-41409 | - |
L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | I3ZR32 | - |
- |
| Bifidobacterium longum NRRL B-41409 | I3ZR32 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE Sepharose column chromatography and hydrophobic interaction chromatography | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-galactose | - |
Bifidobacterium longum | D-tagatose | 36% yield after 6 days of incubation at 35°C | ? | |
| D-galactose | - |
Bifidobacterium longum NRRL B-41409 | D-tagatose | 36% yield after 6 days of incubation at 35°C | ? | |
| L-arabinose | - |
Bifidobacterium longum | L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
| L-arabinose | - |
Bifidobacterium longum NRRL B-41409 | L-ribulose | 11% yield after 2 h of incubation at 35°C | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Bifidobacterium longum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-AI | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bifidobacterium longum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 55 | the enzyme maintains its full activity for 2 days at 25 and 35°C, and over 50% of its full activity for 2 days at 45°C (half-life 52 h). At 55°C, the activity decreases rapidly (half-life 3 h) | Bifidobacterium longum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Bifidobacterium longum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | the enzyme retains over 70% of ist relative activity for 5 days between pH 6.0 and 7.0. At pH 5.0 and 8.0, the relative activity decreases relatively rapidly to less than 40% of the initial activity (half-lives 6 and 28 h at pH 5.0 and 8.0, respectively), after which the decrease rate levels off. At pH 4.0, all activity is rapidly lost (half-life less than 1 h) | Bifidobacterium longum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.012 | - |
D-galactose | at pH 6.5 and 45°C | Bifidobacterium longum | |
| 0.8 | - |
L-arabinose | at pH 6.5 and 45°C | Bifidobacterium longum | |
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